Hi Richard,
I prefer Upright freezers and we have an excellent track record with the Sanyo
VIP Freezer (one of our Sanyo low temp freezers is 15 years old and still
working with no problems).
http://us.sanyo.com/dynamic/product/Downloads/MDF-U33VC52VA53VC73VVIPSeriesBrochure-26928829.pdf
Rega
Hi Jackie, I haven't done a lot of eyes, but mainly sheep lenses. I seemed to
have more trouble with crushing artifact when trimming in- so I tried not to
trim too close and do the closer trimming on the microtome- I also trimmed
before they got too hard from fixing. Tom T
-Original Message
Filtering stains was a long standing habit in our lab. It didn't matter if
the solutions were coming from a stock bottle or reused and filtered into a
clean container.
However, the kind of filter paper is important and something I learned
early on from Lillie and Fullmer's book, Histopat
We use -80 uprights and even with a digital temp readout we have a chart on all
of them (about 20). We are a tissue bank as well as doing research. We have
what we call a freezer farm.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie C
We use Sanyo and Revco uprights with good success. One key ease of use feature
has been the use of front-pull racks for sample box storage. Only one row at a
time needs to be pulled out rather than a whole rack. We have them linked to a
central monitoring system for temperature and power outage
Revco freezers are very good. I prefer uprights for their ease of use (with
racks and seeing the stored samples) and use of doors over stryofoam covers
used for chest freezers.
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie
In my experience the uprights have more frost buildup problems, causing sealing
problems. The chest ones I've had in the lab do less of this.
Laurie
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun,
I need a recommendation for a -80 degree C. freezer for storing tissue
specimens. Do you prefer upright vs. chest? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford
My educated guess would be that the tissues are being dried out prior to being
placed in formalin at time of collection. Surgeons are our biggest histology
problems.
Jackie O'
-Original Message-
From: April P - Assocd
To: histonet
Sent: Thu, Dec 12, 2013 11:24 am
Subject: [Histonet
Bea
Huron Technologies has a large format slide scanner.
http://www.huron-technologies.com/ up to 8 x 6 slides. Most older Aperio
Scanners such as the Scanscope CS or XT can scan 2x3 slides, you just need the
2x3 slide tray, you cannot batch scan its one slide at a time. They did at one
tim
Hi histonetters,
Does anyone know about any automated 2x3 slide scanners, besides Leica? We know
that Leica has one, but does not make any new ones anymore.
Thanks in advance,
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 9
If similar specimens in the processed batch do not have problems, the problems
you are having with those few have to be caused before or during fixation.
Perhaps drying before fixing could be a cause.
René J.
From: April P - Assocd
To: histonet@lists.utsouthwes
To: histonet@lists.utsouthwestern.edu
Date: 12/12/2013
Blount Memorial Hospital in Maryville, TN will have a histology technician
position opening soon.
If you are interested in working for a hospital that cares about its
patients, we may be what you are looking for. The histology laboratory
Need feedback. We are a Derm lab
We are having an issue with some small punches and shaves being crushed or
parched. It does not happen every day and it is maybe one or two when it is
happening. Does anyone have any ideas on what can be causing this issue?
___
Let the eye soak in ice water before cutting for a very long time.
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histon
I have a study coming up where obtaining perfect lens histology is critical.
We do a pretty good job routinely, but since the lens is of interest, I would
appreciate any tricks and techniques that can help us improve our paraffin lens
histology within intact rodent eyes.
Thanks,
Jackie O'
Contact Russ at StatLab. We bought ours from StatLab. Good quality latches
compared to some we bought.
Russell Komae
StatLab Medical Products
Southern California Account Manager
407 Interchange Street | McKinney, TX 75071
t: 310.529.4465 | f: 972.436.1369
rko...@statlab.com | www.statlab.com
>
I beg your pardon!?!
Just joking. Bruce is a brand name. And I don't strip. (Certainly not at my age)
Respectfully,
Bruce Gapinski HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF
(415) 209-6076
Important Notice: This e-mail is intended for th
Lee, we are currently working with a company that is developing a "SmartBx"
mold specifically for prostate cores - it works with a "receiver" for the bx at
the bx suite which holds the bx in a linear trough. Then that "receiver" is put
into an embedding mold with corresponding troughs for perfec
If you don't want to use a knife blade to trim the excess wax, embed at one end
of the mold and use the hot plate to remove excess wax on the other end.
Only problem may be getting a ribbon if one edge of the trim surface is not
straight.
-Original Message-
From: histonet-boun...@list
Goo-gone with a green scrubbie on the tough spots. Then alcohol to clean up
the goo-gone.
--
Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
___
Histonet mailing list
Histone
Speaking of wax on the floors - they did my floors last nite and they are so
pretty I don't think I can drag all the stuff back in that room and go to work.
They look like glass but are not slippery. Don't know what they used.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University
Long thin base molds...I don't think such a thing exists but you could make
some pretty easily. Be innovative!
Look around you and see what already exists out there that would work. Recycle!
I've found plastic containers from packaged foods and items from hardware
stores that could have uses in
I agree with Liz - or a kitchen store. Whatever kind of store I bet you can
make one pretty cheap. I have had our biomed guys here make some things for me
for just a few dollars.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histolo
The kind with the blade was damaging our floors and the blade would break
causing a major safety issue. We just bought a long handled scraper from
Bel Art; it works really well.
http://www.amazon.com/Bel-Art-Scienceware-368290001-Handled-Scraper/dp/B002VBW4N2/ref=sr_1_fkmr0_1?ie=UTF8&qid=1383939732
Treosin from StatLab Medical. RBCs stain a bright orange.
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
From: Patricia
Hello!
I'm looking for a protocol for specific staining of erythrocytes in
paraformaldehyde fixed, paraffin embedded tissue. Is there anything out
there other than H&E?
Thanks for any help!
--
*Patti BourassaSenior Scientist Karos Pharmaceuticals(203) 535-0540, ext
207 <%28203%29%20535-0540
Hi All-
Can anyone tell me where I can purchase histology base molds in a long, thin
rectangular shape? We would like to be able to get more cuts of a prostate
biopsy onto a single slide. We currently are using this shape of a base mold
and having to manually trim excess wax (dangerous and ti
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