Hi Everyone.
I am looking to stain alcohol fixed smears with a group of antibodies.
The antibodies of particular interest are p63, p53 and ki67.
Along with these antibodies I am looking for positive nuclear and
cytoplastic staining antibodies.
If anyone has any experience in staining alcohol
Levi,
I've worked up most of the more common antibodies on alcohol fixed
smears. There are two things to remember here: 1) your specimens are
alcohol fixed, meaning antigen retrieval needs are different, and 2)
they are smears, meaning the cells weren't 'cut open' during microtomy,
which
