Hi,
there is disagreement at the our dept regarding the control slides for IHC, so
far on the control slides there is only writen control, marker and date of
staining (on automated stainer) but there is NOT indicated/writen the ID of
control, however I believe or it's obvious to me that is
I need help with warthin starry stain please. I am a very small lab
trying to save money so I want to make the reagents myself. I have
sigma Aldrich dry reagents and have been making the reagents and
following the instructions correctly and I did get the stain to work
wonderfully when I first
I am also in a small lab and this is how I do our Warthin Starry. I usually
only have 1 or 2 slides to stain at a time and I stain them in a (brand new
never before used for anything) plastic 5-slide mailer. I mix the reagents in
disposable 20 ml beakers. I stain the slides using my
Although many histotechs successfully use the Warhin Starry stain (or so they
say!) I find it the most unreliable and capricious of all stains, so much so
that I stopped using it many years ago and started using the modified Steiner
that stains H.pylory and all Treponema and related bacteria.
Hydroquinone has a short shelf life once opened.
On Jan 14, 2014, at 6:59 AM, Campbell, Tasha M. tmcampb...@fmh.org
wrote:
I need help with warthin starry stain please. I am a very small lab
trying to save money so I want to make the reagents myself. I have
sigma Aldrich dry reagents and
I was wondering about that. Even in its crystal form? I've been trying
to test every possible problem, though, and I am using a kit right now
so I have substituted the kit's hydroquinone in place of mine and used
the rest of my reagents. It still didn't work. So this has to mean
there is
My hydroquonine was received in the lab in 1980 and it works. As long as my
control is positive I am allowed to keep it but then I work in a veterinary
diagnostic lab and am not under all the regulations that those of you in
hospitals are under.
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn
Im sorry Linda! I got myselft added and posted it and I have already
had feedback! Thank you though.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
Dear Histonetters:
Here is a message I am posting from Tasha...she hasn't yet joined the list but
is eager for answers to her question. I will forward answers to her or you can
send them directly to her at
tmcampb...@fmh.orgmailto:tmcampb...@fmh.org
I need help with warthin starry stain
We track shallow/incomplete sections and have found numerous reasons.
Inadequate fixation.
The way a tissue is bisected can cause it to not lay flat in the embedding
mold. For anstance a grossing tech who does a wrist turn kind of slicing
motion and causes an arch shaped edge.
Embedding
Learn IHC staining, Hands - on IHC Lab courseCEU approved; February
27th 28th, 2014 San Francisco Bay area.
For more info contact Maria workshop...@gmail.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Same here.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Tuesday, January 14, 2014 9:53 AM
To: Jennifer
Hi All,
I'm currently recruiting for a new IHC tech with a facility located in San
Francisco, CA. Please find job description below:
IHC Histotechnologist/Histotechnician
Our Client is an exciting, high-growth molecular clinical laboratory located
in the Bay Area (Northern CA), utilizing
Agreed. They can plop it on the block, cut it , stain it but they can't measure
it, dissect it or ink it as those task are considered gross dissection which is
high complexity and the person doing them has to meet CLIA guidelines for high
complexity.
Sent from my iPhone
On Jan 13, 2014, at
I have actually had some success in reducing recuts, and attribute to a couple
of things.
1. Are pathologists are now measured on a case TAT without any
exceptions. (before they could exclude cases for additional
work/stains-(including recuts), held for fixation, decal...). They are
Thank you for the kind responses.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Montag, 13. Jänner 2014 19:56
An: Histonet@lists.utsouthwestern.edu
Betreff: [Histonet]
Hi Leann,
Dependant on the case and how much tissue comes in from the OR, In my many
years...I have on many occasions grossed and placed tissue for FS on chuck, and
performed the frozen section, had it under the scope looking at it prior to the
Pathologist coming in for DX.(why) because we
We, too, had an excess of recuts and deepers. After careful study, it
was found the majority were due to a preference for certain
pathologists, but many were due to poor grossing / poor processing, and
a few were due to tech error with tech error being the least number of
the categories
We
Hi Patricia,
Error rates should not be set by employers. You should have an effective QA
management program and committee even in small practices with the Medical
Director as the Chair. The committee sets and establishes error rate thresholds
with monthly monitors in place to seek
In response to message 4 what is considered the requirements for high
complexity testing for grossing... is that a bachelor's degree with
pathologist guidance or do they have to have a certificate of completion for
being a P.A. (ASCP).
I am out of the office until 01/22/2014.
I will be out of the office from January 13th- January 22nd. I will be
checking emails periodically and will attempt to reply to your message
within 24 hours.
Thank You,
Bruce Palmatier
Market Portfolio Manager
VWR Healthcare
bruce_palmat...@vwr.com
Hi Tasha
I have been performing the Warthin-Starry successfully for nearly 40 years.
Without seeing your method these are a few tips that should be followed.
1. Make sure all solutions are made fresh each time using an acetate
buffer or acidulated water as diluent. I prefer an acetate
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