[Histonet] 1. IHC wet workshop announcement (Workshop IHC)

2014-01-15 Thread Anna Coffey
Does anyone know of any similar IHC courses on the east coast? I'd love to attend this one, but won't be able to swing the travel costs. Thanks! Message: 1 Date: Tue, 14 Jan 2014 08:41:40 -0800 From: Workshop IHC workshop...@gmail.com Subject: [Histonet] IHC wet workshop anouncement To:

[Histonet] FW: Frozen sections (UNCLASSIFIED)

2014-01-15 Thread Bassett, Juan L CTR (US)
Classification: UNCLASSIFIED Caveats: NONE Hi LeAnn, Please note my response below. -Original Message- From: Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 7:39 AM To: 'Michael LaFriniere' Cc: 'stonet-boun...@lists.utsouthwestern.edu' Subject: RE: Frozen sections

[Histonet] RE: Frozen sections (UNCLASSIFIED)

2014-01-15 Thread Michael LaFriniere
Hi Juan, I am not sure of any ASCP guidelines nor jurisdiction over CLIA requirements. It is my understanding all US laboratories have to follow the CLIA set guidelines for performing all laboratory testing. Qualified HT or HTLs can gross complicated (high Complexity) testing under specific

[Histonet] RE: Warthin Starry Help

2014-01-15 Thread Campbell, Tasha M.
Thank you so much. These tips seem very helpful. Could you give me your protocol? Times in temps in the impregnation step. And your measurements for each reagent in the developing solution. I use acidulated water and I usually get the pH at about 3.8. I am staining for h.Pylori. Like I

[Histonet] FISH technique and NBF as fixative

2014-01-15 Thread Nicole Cosenza
I need to do FISH with the universal bacterial probe EUB338. I am not too familiar with FISH. Can we fix the sample with NBF or is 4% paraformaldehyde better? The samples are acellular dermal matrix. A quick lit search shows people using 4% paraformaldehyde, but the histologist that will be

[Histonet] HER2/NEU

2014-01-15 Thread Knutson, Deanne
I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from

[Histonet] RE: HER2/NEU

2014-01-15 Thread Martha Ward-Pathology
That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest.I would be interested in hearing others responses as well. Thank you for asking the question.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center

RE: [Histonet] RE: HER2/NEU

2014-01-15 Thread joelle weaver
Consider the ASCO-CAP guideline and scoring changes for Her2/ FISH, not sure of the timeframe for your trend in increased positives, but my pathologist has indicated that I should expect more positive and equivocal cases, so perhaps related to categorizing of DX, rather performance or clone

[Histonet] RE: Recuts/deepers

2014-01-15 Thread Trini
This is my first time working in a GLP/GMP lab, Ive only been here for 6 months. We cut a variety of tissue. At trimming is where they decide what tissue to go in what cassette and so one cassete could have 4-6 different types of tissue. And on the other hand we do osteoinduction studies, and

[Histonet] Cryostat Decontamination

2014-01-15 Thread Abbott, Tanya
I am looking for a procedure and product to use for decontamination after a potential TB case, help please! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net

[Histonet] 2014 CPT Changes

2014-01-15 Thread Susie Hargrove
Hello, I know this subject had been looked at many times. I just want to see if I am correct before I start doing anything different. 1. 88342 , not much change . We can still just bill once per site, per case. This is per single antibody per Specimen. If I have a case with two parts and

[Histonet] Cryostat

2014-01-15 Thread Abbott, Tanya
Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the

[Histonet] Do I have to destabilize MMA?

2014-01-15 Thread abtdhu
I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to