[Histonet] wearable formalin monitors

2014-02-19 Thread Finlay Finlay
Hello Just wondering what everyone's experience is with different wearable formalin monitors. I'm looking at the AirChek ones from Leica at the moment. Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical,

[Histonet] RE: wearable formalin monitors

2014-02-19 Thread Morken, Timothy
Our formalin and xylene badges are from Advanced Chemical Sensors, Boca Raton, FL. Our Saftey group supplies these to use for monitoring. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message-

[Histonet] aqueous mounting medium

2014-02-19 Thread Jennifer MacDonald
Does anyone have a good recipe for aqueous mounting medium? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] To those attending Tri-State in Rochester MN

2014-02-19 Thread Beth Brinegar
Just giving a heads up to those needing hotels for the TriState Symposium 4/30-5/2 that the Super 8 is located just 1.1 miles down S Broadway from the Double Tree Hotel (where the symposium is being held) and is only $50.49 a night (if booked early) instead of $132.00 a night (at the Double Tree).

Re: [Histonet] aqueous mounting medium

2014-02-19 Thread Hans B Snyder
Hello, I have one for ORO but not for IF. If someone has one for IF, I would be interested also. Thank you Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com On Feb 19, 2014, at 12:27, Jennifer MacDonald jmacdon...@mtsac.edu wrote: Does anyone have a good recipe for

Re: [Histonet] aqueous mounting medium

2014-02-19 Thread Caroline Miller
I use prolong gold from life tech (formerly molecular probes and now part of the world dominating thermofisher) without Dapi as I do that separately, for IF and aquaslip from American master tech for non IF aqueous mounting Good luck! Caroline Caroline Miller Gladstone Institutes

[Histonet] Billing

2014-02-19 Thread Abbott, Tanya
Does anyone bill for a technical component for send outs? Meaning, billing for supplies, time it takes to process sendout/receive them back in, etc? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax

Re: [Histonet] aqueous mounting medium

2014-02-19 Thread Mehmet Fatih BOZKURT
I'm using modified protocol. I'm storing it at 4C. *Aqueous Mounting Medium (glycerol gelatin)* *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5 gDistilled water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol (carbolic acid) 5 ml5 ml Mix gelatin

[Histonet] Used Equipment

2014-02-19 Thread McKenzie, Emily
Hello Histoneters!! Our facility is upgrading our IHC process. We are currently in the market for a used linear stainer (for hydrate and dehydrate) and/or a used coverslipper (glass). These items need to be fairly small since we don't have a lot of room to put equipment. If there are any

[Histonet] Job Opportunity at Georgetown University Histopathology and Tissue Shared Resource Lab

2014-02-19 Thread Anna Coffey
We have a new histology technician position that has just opened in the Histopathology and Tissue Shared Resource lab at Georgetown University's Lombardi Comprehensive Cancer Center. We're looking for someone with some lab experience (B.S. in biology, chemistry, or related field preferred) who is

[Histonet] Dried out liver bx's

2014-02-19 Thread SARAH GIBSON
We are having problems with some of our small liver bx looking burned and dry. Does anyone have any suggestions on ways to fix this problem and any techniques while sectionly to get a better quality section. Thanks for any help. Sent from my iPad ___

[Histonet] HIRING PT and per diem DELRAY BEACH FL

2014-02-19 Thread Delray Beach Pathology Kari Simeone
Hi Histonetters! We are still interviewing for a part time tech here in our very busy east Delray FLORIDA Dermatology Lab (upwards of 80k cases/year). This is a permanent part time position with full time growth potential. PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!! Email your resume to

Re: [Histonet] Dried out liver bx's

2014-02-19 Thread Mehmet Fatih BOZKURT
Hello, You can try less alcohol fixation and before cutting you can put blocks in wet-ice box. On Wed, Feb 19, 2014 at 9:46 PM, SARAH GIBSON sara...@hotmail.com wrote: We are having problems with some of our small liver bx looking burned and dry. Does anyone have any suggestions on ways to

[Histonet] Cytolyt Solution

2014-02-19 Thread Rolly Perez
Anyone knows how to make Cytolyt Solution? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mesothelioma Control

2014-02-19 Thread Coppin, Margaret
Hello everyone, I am on a desperate search for a good mesothelioma control. Can anyone out there help? Either a block or even just a few unstained slides would be great! Thank you. Margaret G. Coppin, HT (ASCP) Technical Supervisor, Immunohistochemistry Integrated Oncology and Genetics Group

[Histonet] RE: Cytolyt Solution

2014-02-19 Thread Walter Benton
Hologic Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines.

[Histonet] Validating New IHC antibodies

2014-02-19 Thread Abbott, Tanya
What's everyone's go to # for Pos/Neg slides when validating new IHC antibodies? (Not counting Her2 and ER/PR, of course!) Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email:

[Histonet] affymetrix Anti-Mouse F4/80 antigen purified

2014-02-19 Thread Jennifer Leigh
 All-       Has anyone worked with the affymetrix Anti-Mouse F4/80 antigen (catalog #14-4801) in paraffin embedded tissue? The spec sheet indicates that proteinase treatment is needed. Any insight for working with this particular antibody would be very much appreciated.

RE: [Histonet] Validating New IHC antibodies

2014-02-19 Thread joelle weaver
minimum 10, usually 25 for binary scored-diagnostic markers. More in many cases if ASR. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tanyaabb...@catholichealth.net To: histonet@lists.utsouthwestern.edu Date: Wed, 19 Feb 2014 20:12:07 + Subject: [Histonet] Validating New IHC antibodies

RE: [Histonet] Dried out liver bx's

2014-02-19 Thread Tony Henwood (SCHN)
Hi Sarah, 90% of the time it is a collection issue. Do they leave it on filter (or any other absorbent) paper before placing in fixative. OR, heaven forbid, are they putting it on one of those alcohol wipes they use for disinfecting skin before biopsy? Regards Tony Henwood JP, MSc, BAppSc,

[Histonet] Xylene Resistant Labels

2014-02-19 Thread Schaundra Walton
Hey Histonetters! We are looking to make some cost effective efficiency changes in some of our outlying labs. We have one small lab currently hand writing slides and then printing paper labels and relabeling slides after staining. Does anyone know of xylene resistant labels that could be

RE: [Histonet] Xylene Resistant Labels

2014-02-19 Thread Elizabeth Chlipala
We use a printer that we purchased from General Data and their labels and they created a specialized software for us since we are a research lab. The labels work great in our hands. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303)

Re: [Histonet] Dried out liver bx's

2014-02-19 Thread Campbell, Tasha M.
Rough cut then soak in your waterbath for a minute or so then put back on ice to cool. Then section. U should get a beautiful ribbon :) Tasha Sent from my iPhone On Feb 19, 2014, at 2:49 PM, SARAH GIBSON sara...@hotmail.com wrote: We are having problems with some of our small liver bx

Re: [Histonet] aqueous mounting medium

2014-02-19 Thread Lee Peggy Wenk
to be used for what? - Lipids - ORO, Sudan black B? - Immunofluorescence - which dye? Different needs, different requirements. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Jennifer MacDonald Sent: Wednesday, February 19, 2014 12:27 PM To: histonet@lists.utsouthwestern.edu

Re: [Histonet] aqueous mounting medium

2014-02-19 Thread Jennifer MacDonald
Enzyme histochemistry On Feb 19, 2014, at 6:42 PM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: to be used for what? - Lipids - ORO, Sudan black B? - Immunofluorescence - which dye? Different needs, different requirements. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From:

Re: [Histonet] Dried out liver bx's

2014-02-19 Thread Jennifer MacDonald
We had the same problem with GI biopsies. The problem was always with the same tech. She was blasting the blocks with freezing spray and they were freezer burnt. On Feb 19, 2014, at 11:49 AM, SARAH GIBSON sara...@hotmail.com wrote: We are having problems with some of our small liver bx