[Histonet] Disposable blade holder

2014-02-27 Thread Mike Pence
Need your help! I am looking for a disposable blade holder for a Microm HM355S microtome. A new one is over $2000 and I just need the front part of the blade holder (which they don't sell separately). I would buy a used (but in good condition) so I would have some extra parts. If anyone has on

[Histonet] microscope information

2014-02-27 Thread Freeman, Lisa*
I am currently looking for a microscope to use at the sectioning stations. We have to locate tissues such as parathyroid glands and mammary glands at sectioning, as well as general QC. Any opinions on which is the best for this purpose? Thank you, Lisa Freeman, HT Histology Supervisor

[Histonet] RE: microscope information

2014-02-27 Thread Elizabeth Chlipala
Lisa We purchased some Leica Student scopes for this purpose one for each of the sectioning stations. They are Leica DM750's. They are really nice, we do have a couple other microscopes we purchased previous to the DM750's but they were not as nice as these. Liz Elizabeth A. Chlipala, BS,

Re: [Histonet] manual tissue processing protocol

2014-02-27 Thread Rena Fail
Hi, One mm thick is tiny, 2 mm small 1 cm is rather thick. Since you are manually processing run one sample to test your protocol or 2 if you want to see the difference between the shorter method and the longer. *protocol. .Complete dehydration is dependent on the volume, type of tissue and

Re: [Histonet] RE: microscope information

2014-02-27 Thread Robin
Sent from my iPhoneohiuB On Feb 27, 2014, at 12:57 PM, Elizabeth Chlipala l...@premierlab.com wrote: Lisa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Histonet Digest, Vol 123, Issue 29 . Input on Sakura E150 Purchase (margaret chadwell)

2014-02-27 Thread Magda G
Hello, We used to have one of E150, but during our last PM our technician told me that they no longer make any parts for it so if it will break it may be impossible to be fixed. Maggie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] microscope information

2014-02-27 Thread James Watson
If you drop the condenser down so Koehler illumination is not aligned, this give you a pseudo phase contrast. You can see many structures in tissue due to the different density of the cells. So I would recommend a microscope that has a condenser that can be moved up and down. We have 2 old

[Histonet] New GHS labels

2014-02-27 Thread Cheri Miller
I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar

[Histonet] RE: New GHS labels

2014-02-27 Thread Vanessa Perez
The global hazard chemical labels are for shipping purposes only on the bottles from the companies. The reagents made for in the lab that are put in containers can still use the same hazard labels you use now with the diamond. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746

[Histonet] RE: New GHS labels

2014-02-27 Thread Blazek, Linda
Here is what I have in my notes for the new standards. I think you're probably safe with what you currently have as long as you've don't the training that should have been done by Dec 1st 2013. The first compliance date of the revised HCS is December 1, 2013. By that time employers must have

[Histonet] RE: New GHS labels

2014-02-27 Thread Stacy McLaughlin
Per OSHA: As of June 1, 2015, all labels will be required to have pictograms, a signal word, hazard and precautionary statements, the product identifier, and supplier identification. A sample revised HCS label, identifying the required label elements, is shown on the right. Supplemental

[Histonet] Disposable Blade Holder

2014-02-27 Thread Mike Pence
Need your help! I am looking for a disposable blade holder for a Microm HM355S microtome. A new one is over $2000 and I just need the front part of the blade holder (which they don't sell separately). I would buy a used (but in good condition) so I would have some extra parts. If anyone has on

[Histonet] RE: New GHS labels

2014-02-27 Thread Cheri Miller
Thank you all for your help. Our Safety Officer quit and I don't see anything except her notes from the seminar. I will pass this on to the new safety person. Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117

[Histonet] Z

2014-02-27 Thread Robin
Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Email list

2014-02-27 Thread Jan Shivers
Stephanie, et al Oops, the previous email in this string was an answer on how to unsubscribe from the Histonet. I copied and pasted the link from my own information email, not realizing that it contained MY Histonet email address on the end. Please DISREGARD the link (or delete the link

Re: [Histonet] manual tissue processing protocol

2014-02-27 Thread Kim Donadio
I agree with Renee here about testing one before. However those are thick and I'm supposing your fish have scales? This also might slow the process down. My best guess is you're going to need a much longer processing time. And since you don't have vacuum I personally think that thickness is

Re: [Histonet] manual tissue processing protocol

2014-02-27 Thread Tyrone Genade
Thanks On Thu, Feb 27, 2014 at 5:50 PM, Kim Donadio one_angel_sec...@yahoo.comwrote: I agree with Renee here about testing one before. However those are thick and I'm supposing your fish have scales? This also might slow the process down. My best guess is you're going to need a much longer

[Histonet] negative control for FLI-1

2014-02-27 Thread Pathology-Histology Sr. Supervisor
Dear All, We are looking negative control for FLI-1, as positive control we are using (PNET) on paraffin section successfully. Our pathologist gave us Wilm's tumor and IDC for Negative control but it not work good. Thanks Muhammad Tahseen Sr. Supervisor Histology SKMCHRC Lahore Pakistan