Is this a tape coverslipper? If so, you do have minute traces of water
carryover to your xylene. If there is even the hint of pink in you last
dehydrant before xylene, you will get that cornflake artifact. Acetone wont
help, because water still be present. Increase number of absolute alcohol
You will also see the cornflaking if your tissue is lifting off of the slide at
all. We used to get this more often on hard, decal specimens than on other
specimens. We used the film to coverslip. If you remove the film from the
problem slides and recoverslip conventionally with extra
Good morning Histonet Folks,
I am hoping one of you will help me. I am in the process of optimizing
an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark
XTstainer and I am not thrilled with the results I am getting. I have tried the
usual adjustments and the
We also have recently started to see this artifact more than ever before, and
nothing in our process has changed. We have tried everything to correct to no
avail. Wonder if it is possible to be a change in some type of supply, either
xylene or coverslipping film. Something has changed but am
We had this problem several years ago. We were using the sakura tapes
with the coverslipper.
We did the following:
Last three alcohols were changes frequently.
Slides should be not dry when loading on coverslipper.
If you could load two racks at a time, only load one. By this way the
Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing
this as well out of the blue..haven't changed a thing.
Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email:
This is our protocol for the Ultra; maybe it will help.
64 CC1, 32 incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4. This is
with UltraView DAB detection. We use tonsil as well however we validated with
HD, LN, GI, tonsil, etc.
Good luck!
Linda A. Sebree
University of Wisconsin Hospital
Long time and high volume user of the Sakura Tape. Check the Xylene drops. We
coverslip thousands of slides daily and have seen no isssue with the tape lots.
Our experience shows some cornflaking when the slide does not receive enough
xylens to wet the slide properly.
William DeSalvo, BS
We have changed lots of tape and still are having this artifact.
Lisa LaVigne CT, MB (ASCP)
Pathology Manager
St. Peter's Health Partners
315 S. Manning Blvd.
Albany, NY 12208
Phone: 518-525-5274
Fax: 518-525-6750
Email: lisa.lavi...@sphp.commailto:lisa.lavi...@sphp.com
I just wanted to send a reminder that this Friday, March 15th is the
registration deadline for the NJSH Spring Meeting planned for April 5th in
Mount Laurel, NJ. There will be four 90 minute seminars entitled Respiratory
Histopathology, Pathology of the Urinary Tract, Animal Model Development
In a continuing effort to limit the volume of reagent used in each step of a
manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and
without barriers.
The results were not stellar with the barrier slides - the reagent still
escapes. We dewax with hot detergent (may be a
Dear Histonet,
A colleague in Manicaland Province, Mutare, Zimbabwe is in need of a
thermocycler if anyone has an old one that they would be able to donate.
The colleague is the Coordinator for Laboratory Training at Africa
University.She and the university work to provide opportunities for
We had this issue when we were using the tape from Mercedes Medical and went
back to
Sakura tape and it went away. It could also be associated with humidity.
STP
TJUH
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Why doesn't someone call Kellogg's ?
On Mar 13, 2014, at 12:10 PM, Beth Cox bethc...@gmail.com wrote:
Sharon other Histonetters,
Cornflaking is literally microscopic air trapped under the coverslip. It
doesn't have anything to do with poor dehydration and trapped water. Thus
it can
Sharon other Histonetters,
Cornflaking is literally microscopic air trapped under the coverslip. It
doesn't have anything to do with poor dehydration and trapped water. Thus
it can be caused by partial drying out before the coverslipping.
The things you need to look at to eradicate the problem
Good afternoon all,
I was just curious about how your institutions handle GI biopsies,
specifically how many slides you cut off the bat. We presently cut 2 levels on
each GI biopsy block, but I'm hearing that more and more places only cut 1
slide per GI biopsy block. Please share what
We are all putting out what worked for us. You should try some of these
suggestions and see what works for you. The suggestion for improving poor
dehydration was suggested by technical support from Sakura. Cornflaking
was irritating so many of our pathologist, we had to get technical
We do 3 levels on one slide.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is
We actually cut 2 slides with 4 levels 2 on each slide. Out pathologists were
unwilling to only
cut one slide
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As a long time user of film (though sadly, no longer) we used to see
what sounds like this artifact when the Xylene dispenser was not
dispensing enough xylene and the slides were not wet enough. It drove
us crazy until we realized that through the years, the xylene dispensing
knob had become very
I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it
stains great on tonsil and kidney tubules.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: lseb...@uwhealth.org
To: cda...@che-east.org; histonet@lists.utsouthwestern.edu
Date: Thu, 13 Mar 2014 16:19:11 +
CC:
3 levels - 1 slide
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
imhype...@aol.com
Sent: Thursday, March 13, 2014 2:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI Biopsies
Good
are you looking for the stats in the notes for ANP. 22970 (2012) ?
overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower
average 20-35% in post menopausal) , lower in well differentiated tumors...etc.
I sure CAP can also send or repeat them to you if you prefer to call
sorry , saw the Predictive, missed FISH - getting ready to do this myself. I
would just call CAP, if I get to do that soon I will send along what
information they supply.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: joellewea...@hotmail.com
To: tbr...@holyredeemer.com;
Three levels one slide for all biopsies. Colon polyps one section.
Sent from my iPhone
On Mar 13, 2014, at 11:29 AM, imhype...@aol.com wrote:
Good afternoon all,
I was just curious about how your institutions handle GI biopsies,
specifically how many slides you cut off the bat. We
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician for its'
Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin
and frozen sections), IHC staining
Hello Histonetters,
Is anyone currently or in the past had experience operating the Leica
CM3600 Cryomacrotome XP? I am looking for input on using this instrument.
Thanks,
Tina
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