RE: [Histonet] What is a level?

10 turns of the wheel is fine for most tissue however we often get FNA's that are so tiny that several turns of the wheel and you will be completely through it. It is often a judgment call by the tech. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] fixed fecal smears for CAP parasitology survey 2014 p3

Hi All, We have received two fixed fecal smears from microbiology section ( CAP parasitology survey 2014 p3,)for trichrome or iron-Hematoxylin staining procedures. Would I adopted any kind of trichrome or iron Hematoxylin or some specific ? Thank, Muhammad Tahseen SKMCHRC Lahore

[Histonet] RE: Frozen section PPE

Our 2 cents. For performing frozen sections, we require gown and gloves. If there is a possibility of a infectious granuloma then we add goggles and a mask. Also, we never allow spray coolant in the cryostat as it has the probability of aerosolizing any nasty bugs lurking in the cryochamber.

[Histonet] RE: what is a level

Our 2 cents: What is a level? A level is how you've defined level in your SOP. In this lab, we use the words level and recut interchangeably. We define a level as 40 microns deeper than the previous section. If the pathologist wants to see every section of tissue cut without skipping any

Re: [Histonet] IHC wash with dH20 / follow-up

Remember you are doing them by hand do you can manually place reagent where you want it. Buffer would be necessary on a machine if for nothing else other than as a surfactant to insure all corresponding reagents spread evenly on the slide. I just wanted to add this note in case some did not

[Histonet] RE: what is a level

It can be confusing because people use different words to describe, we call picking up every section serial sectioning and the distance between sections for levels is determined by the requestor in our case, so they might ask for six more levels to be cut with 50 microns thrown out between each

[Histonet] Thrombomodulin positive controls

Hi histoland.anyone have any tissue control blocks positive for thrombomodulin that u can part with. We would appreciate it tremendously. Susan Memorial Hospital Belleville. Illinois. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] IHC wash with dH20 / follow-up

I may be wrong but I think that the: Electrostatic benefit is imparted by the salts in the buffer The surfactant benefit is imparted by the detergent -Original Message- From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] Sent: Tuesday, March 18, 2014 12:15 PM To:

Re: [Histonet] IHC wash with dH20 / follow-up

Buffer in of itself acts as a surfactant on the tissue sections a wash buffer such as a tris(TBS)has salts in it. Saline is a salt solution as you probably know. I'm just giving my opinion as to the benefits of using a buffer but more importantly for anyone out there using a instrument for

[Histonet] ER and PR Controls

Lately I have been reviewing our controls on many of our IHC stains. As I was reading a CAP article it caused me to question our ER and PR controls currently being used. Our control has two pieces of tissue in it. One is a breast tumor positive for ER and PR and the other is a breast

[Histonet] ERG and PTEN antibodies

Does anyone out there have any control slides or blocks they can spare for ERG and PTEN. Our docs want to bring in this antibody, but we don't send out our prostate biopsies for these tests so we don't have any for sure positives for these antibodies. Thanks!!! Vanessa Perez Garcia Pathology

[Histonet] RE: fixed fecal smears for CAP parasitology survey 2014 p3

Hi Muhammad I have done many of these over the years. You want Gomori's one step Trichrome for protozoa etc or Heidenhain's Iron Haematoxylin. They should be easy to google. If you have any problems let me know and I can send some protocols. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief