Good Morning Histonet!
We have had a new opportunity open with a world leading manufacturer of
histology products. Our client is growing and searching for a histology
professional to join their team on the North East ideally based in Northern
New Jersey or New York City. This role will work
For those who have written off list for the Churukian stain procedure, I've
included it here. It is also sold in the USA in kit form by Polyscientific (no
plug here, just stating)
PRINCIPLE:
Fungi and Pneumocystis organisms are oxidized using periodic acid and then
impregnated with ammoniacal
Hello everyone,
When it is working correctly, our tissue processor moves a basket of
tissues through 10 reagent containers. Last night it malfunctioned and
suspended the tissues above the 80% ethanol container. The tissues were
high and dry for ~ 9 hours (!) until I placed them into 70% ethanol
In my experience, periodic acid will not help you demonstrate histoplasma.
Chromic acid should be the preferred oxidizer if histoplasmosis is suspected.
I have had good results with periodic acid for pneumocystis and aspergillus,
but not histoplasma.
Ashley Troutman BS, MBA, HT(ASCP)QIHC
Carla,
I don't think you can be a real histotech if something like this has never
happened to you!
Have you figured out the cause of the malfunction?
There are recipes that you can find online and probably on HISTONET for
solutions to soak your tissues in to rehydrate them. You might
Hello Histonet,
We have been using Lab Vision's DAB away to clean our Autostainer 360. However
it seems a little wasteful to buy something that we can't use up before it
expires. Do any of you have any exerpience with lower cost alternatives?
Thanks,
Tom
Hi All,
Anyone have a good recommendation for a reliable paraffin dispenser? The spigot
keeps clogging on the one we have now.
Thanks,
Michele Margiotta-Watz
Histology Supervisor
BMHMC
101 Hospital Rd.
Patchogue, NY 11772
631-654-7192
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This e-mail and any files transmitted with it
Hello Histonetters,
Does anyone in Histoland have an off market source for the metal tissue
processing baskets used in the Sakura VIP processor? I need to purchase
several of the 150 cassette capacity and several of the 75 cassette capacity.
They are super expensive directly from Sakura.
Not since 1978. (Sorry - couldn't resist - Happy Friday!)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject:
Oh where is the like button. :)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 06, 2014 12:22 PM
To: Conway, Carla; histonet@lists.utsouthwestern.edu
Subject: RE:
I recommend a slide incubator and a metal cup or two. The paraffin dispenser we
purchased seem to be a relicensed commercial coffee pot. The spigot keeps
clogging because they did not design it in a way that it would stay warm so,
the paraffin cools in it and clogs.
Cassie D.
Michele
Yet another reason to go microwave. This can't happen.
- Original Message -
From: Shirley A. Powell powell...@mercer.edu
To: Bill O'Donnell billodonn...@catholichealth.net, Carla Conway
cmcon...@usgs.gov, histonet@lists.utsouthwestern.edu
Sent: Friday, June 6, 2014 12:53:07 PM
My advise is to call Sakura and schedule a maintenance visit. The rotary valve
may be acting up.
René J.
On Friday, June 6, 2014 1:53 PM, Mike Tighe mti...@trudeauinstitute.org wrote:
I am having a problem with our tissue processor (Tissue Tek 3000). During
processing the first reagent
I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that
needs wiped clean. Clearly it's not ready the fluid level in station 3 and
returning it back to 1 for safe keeping.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
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