[Histonet] rolling sections

2014-06-12 Thread Roberta Horner
I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp

[Histonet] RE: rolling sections

2014-06-12 Thread Helen Fedor
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

Re: [Histonet] RE: rolling sections

2014-06-12 Thread Pam Marcum
That is how we do it also.  Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.  If they are doing DNA or RNAase ou may need to wear

[Histonet] RE: rolling sections

2014-06-12 Thread Martha Ward-Pathology
We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890  

[Histonet] RE: rolling sections

2014-06-12 Thread Bernice Frederick
True. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From:

RE: [Histonet] RE: rolling sections

2014-06-12 Thread Truscott, Tom
Hi Roberta, Check with those requesting to see if you can cut the equilivant but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are lots easier to get into the tubes. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Please remove me

2014-06-12 Thread Del Phillips
Please remove me ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Processors again

2014-06-12 Thread anita
Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. Thanks a bunch!!! Have a great day!!! Anita Dudley Providence Hospital Mobile, Al.

RE: [Histonet] Please remove me

2014-06-12 Thread Weems, Joyce K.
Hi Don, You must do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Thanks and best wishes! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342

Re: [Histonet] rolling sections

2014-06-12 Thread Lee Peggy Wenk
Try changing the angle of the knife blade, so that the clearance angle (angle between the knife blade and the block face) is larger. In other words, tip the top of the blade towards the block more. If there are numbers on the side of the knife holder, you want to move it to a larger number

RE: [Histonet] Processors again

2014-06-12 Thread Marcum, Pamela A
We have 4 Excelsiors and love them. We also have one Leica ASP300 and it is a great workhorse for us. I think it just depends on your lab and what you like or what serves you best. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Processors again

2014-06-12 Thread Ronda Mire
I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita azdud...@hotmail.com wrote: Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and

[Histonet] Vantange/ block printer

2014-06-12 Thread Amber McKenzie
For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks!

Re: [Histonet] Vantange/ block printer

2014-06-12 Thread Pam Marcum
Thermo also has a cassette printer that works well.  We have t he cassette printer (6 hoppers per unit) and slide writers that work for us.  Pam Marcum - Original Message - From: Amber McKenzie amber.mcken...@gastrodocs.net Cc: Histonet histonet@lists.utsouthwestern.edu Sent:

[Histonet] RE: Vantange/ block printer

2014-06-12 Thread Morken, Timothy
Amber we got the Leica inkjet printer (6 actually). The 2D code reads very well. It prints at 5-7 seconds per cassette (depends on how much text you cram on there!). It is about the fastest printer out there. Data General is about the same speed, if you print with a reverse image ( black

Re: [Histonet] Processors again

2014-06-12 Thread Sue
I used the Logs quick processor and it was really nice for small biopsies, no experience with their routine processor.  Have 6 Excelsior's and love them. STP TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Formalin in operating (surgery) rooms

2014-06-12 Thread Candace J. Wagner
Hello all out in Histoland, I had a surgery tech ask me if there was a specific amount of formalin allowed in the surgery rooms. I could not find anywhere any documentation on a specific amount. We supply our surgery dept. with the formalin they need, usually about 2 gallons in each room now,

[Histonet] (no subject)

2014-06-12 Thread awill.imdpath...@yahoo.com
Please remove me ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Formalin in operating (surgery) rooms

2014-06-12 Thread Podawiltz, Thomas
We do not allow any formalin in the surgery rooms. They come to the Histology department to pre-fill their containers since they do not a fume hood in the OR. Those containers go into a utility room and a scrub tech will leave the surgical suite to place the collected specimen in the

Re: [Histonet] RE: Formalin in operating (surgery) rooms

2014-06-12 Thread Sue
We do not allow formalin in the actualy OR, yet it is in the Ultrasound Suites.  There is a holding area outside the OR with the formalin, S Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] (no subject)

2014-06-12 Thread Weems, Joyce K.
Hello, You will need to do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342

[Histonet] RE: Vantange/ block printer

2014-06-12 Thread Vanessa Perez
We use one from general data. Seems to work well with us, the only thing is you have to change the hopper when you want to change the cassette color. We have learned to just batch all the specimens together that have same color cassette. I believe you can get another model that has the

[Histonet] RE: Histonet Digest, Vol 127, Issue 9

2014-06-12 Thread Hugh Luk
Hi Ronda Mire, Your best bet is to find someone who discarded an old VIP and has kept their baskets when they purchased new machines, to sell you theirs. Sorry I don't fit this criteria anymore. We have thought about replacement parts too, but have not found baskets that work as well as the

[Histonet] IHC Supervisor - Dallas

2014-06-12 Thread Pat Patterson
We have a current opening for an experienced IHC Supervisor to oversee the overnight operations of our high volume, high quality immunohistochemistry laboratory. ProPath is a progressive, CAP accredited, high-volume pathology practice located in Dallas, Texas. Responsibilities include slide