RE: [Histonet] need recommendations on working alpha synulcein IHC for human tissue

I would also be interested in what Alpha synuclein Abs people are using for IHC Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From:

[Histonet] Can acetic acid in fixative affect fungal staining by PAS?

Hello, I fixed skin/muscle and gill tissues for 48 h in Dietrich's fixative (30 mL 95 % ethanol, 10 mL formalin, 2 mL glacial acetic acid and 58 mL deionized water) and used the Hotchkiss-McManus PAS protocol to stain *Saprolegnia *fungi. The fungi are light green from the counterstain and not

[Histonet] BrDU

Hi Histonetters Can anyone recommend a good anti-BrDU antibody and protocol Thanks Happy Summer!! This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is

RE: [Histonet] Eosin Leaching

I have seen this happen with high levels of Isopropanol in alcohols after the eosins, I switch to regular reagent alcohols and seemed to eliminate the bleeding of the eosin. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield

[Histonet] RE: BrDU

Apologies, I meant to add for FFPE muse/rat tissue TX's -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, June 24, 2014 11:12 AM To: (Histonet@lists.utsouthwestern.edu)

Re: [Histonet] Slides for Thermo Slidemate

Cancer Diagnostics has an Autofrost clipped corner that completely eliminated our slide jamming issues and doesn't smear. We have 7 of these printers and have had no issue. They have multiple colors,and also have a good silanized Autofrost IHC slides (we get the 90 degree corner for our machines)

[Histonet] Re: Slides for thermo Slidemate

Those of you having trouble with slides in the Slidemate, please respond to me privately and let me know which ones are causing trouble for you. Thanks! Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752

[Histonet] RE: BrDU

Hi Luis, We are using the BrDU In-Situ Detection Kit #551321 from BD Biosciences with good luck. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

[Histonet] Posting for a HIstonet member- Position available

If you're looking for a friendly environment, a new state-of-the-art facility and an employer of choice, SPL is the place for you!! We currently have several openings: * Histotechnicians/Histotechnologists Histo candidates should be an HTL or HT (ASCP) or equivalent. Primary

[Histonet] Histotechnologist Opening Immediately - Plum Island, USDA

A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old

[Histonet] BrDU

Hi, I use Sigma's B2531 with 1 hr pretreatment in 1N HCl at 40 deg (in a coplin jar in my waterbath) then 15 min Trypsin (don't forget the CaCl2) at 40 deg. I detect it witb Envision+ but any mouse IgG would work fine. Amos Message: 15 Date: Tue, 24 Jun 2014 15:12:01 + From: Chiriboga,

[Histonet] Histotechnologist Job Opening Immediately - Plum Island, USDA

A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old