RE: [Histonet] On the lighter side...

2014-08-11 Thread Susan.Walzer
For me 36years..will not be 37, I retire in 3 weeks! Off to visit my son and family in England! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 11:01 AM To:

RE: [Histonet] On the lighter side...

2014-08-11 Thread Edwards, Richard
Sniffed my first formalin and saw first post-mortem November 1965. Richard Edwards Leicester U.K.

Re: [Histonet] On the lighter side...

2014-08-11 Thread Michael Ann Jones
25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, Edwards, Richard

[Histonet] Anti MMP-9

2014-08-11 Thread Johns, Laura [ETHUS]
Hello, Can anyone recommend an antibody for MMP-9 that works in FFPE porcine tissue? Thanks, Laura ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] On the lighter side...

2014-08-11 Thread Ingles Claire
Old histologists never die, they are just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To:

Re: [Histonet] On the lighter side...

2014-08-11 Thread Michael Ann Jones
Oh, I ment to say “Lots of tenure here and lots of brain cells here” meaning this website and all histotechs here, not myself. Obviously I don’t have many brain cells left If I made it look like I was talking about myself. . .sheesh. . Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444

[Histonet] Ventana Symphony

2014-08-11 Thread Fawn Bomar
Can anyone using the Ventana Symphony share its pros and cons with me? Would you recommend this stainer? Thank you Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended

[Histonet] Need microvascular (MVD) staining and quantification of tumro vasculature.

2014-08-11 Thread Hans B Snyder
Hello All, I have a customer who needs microvascular (MVD) staining and quantification of tumro vasculature, where staining is typically done using CD31. Is there anyone who can do this and willing to forward their information to me? Thank you Hans B Snyder Histologistics 60 Prescott Street

RE: [Histonet] On the lighter side...

2014-08-11 Thread Shirley A. Powell
I agree. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old

[Histonet] IHC Steam Strips:

2014-08-11 Thread Jb
Does anyone use IHC steam strips in their decloaking chamber? The question is do you run a new steam strip each run and log each individual run w/it's own steam strip? Thank you, Sent from my iPhone ___ Histonet mailing list

[Histonet] Bcl-2

2014-08-11 Thread Heather Knight
Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight

RE: [Histonet] Are Paraffin Blocks Biohazard

2014-08-11 Thread Michael LaFriniere
Dawn, The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive formalin fixation and routine processing protocols, the CDC web site has additional information, In my laboratories I put all blocks in hazardous waste for incineration disposal. It is not that costly just to

RE: [Histonet] On the lighter side...

2014-08-11 Thread Mark Turner
Saves on embalming costs Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC   -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles

RE: [Histonet] On the lighter side...

2014-08-11 Thread Bernice Frederick
Or we'll live forever. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From:

[Histonet] Weight Loss/Weight Gain Decal

2014-08-11 Thread Wait, Trevor Jordan
Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I

Re: [Histonet] Weight Loss/Weight Gain Decal

2014-08-11 Thread Jennifer MacDonald
I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying

RE: [Histonet] Weight Loss/Weight Gain Decal

2014-08-11 Thread Wait, Trevor Jordan
Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From: Jennifer MacDonald

[Histonet] RE: Histonet Digest, Vol 129, Issue 17

2014-08-11 Thread Mayer,Toysha N
Egads, I feel like a baby! 20 years registered, 3 yrs unregistered. By the way, I did see a former colleague with her ASCP certificate in her office, and the oldest sticker is from the year I was born. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator

[Histonet] RE:On the lighter side...

2014-08-11 Thread Mayer,Toysha N
Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as long as I got a marketable skill along the way. The best advice I've ever gotten. Thanks Maria!!! (my mother) Sincerely, Dr.Toysha

Re: [Histonet] RE:On the lighter side...

2014-08-11 Thread Barry Rittman
Well fixed in the histological sense I hope. Barry On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N tnma...@mdanderson.org wrote: Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as

[Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test

2014-08-11 Thread gayle callis
Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical

[Histonet] Consult for taking abs from RUO to IVD

2014-08-11 Thread Patsy Ruegg
Colleagues, I am consulting with an antibody vendor who needs a consultant to help them take their abs from RUO to IVD. If you know anyone who does this type of work you could recommend would you please have them contact me directly and I will pass their contact info on. Best regards, Patsy