[Histonet] Myophosphorylase

2014-08-15 Thread Celebre Julia
Hello Histoland, This is a two part question for all those who do muscles. 1.Is anyone having problems ordering glucose-1-phosphate? 2.Does anyone have a method for myophosphorylase that doesn't require glucose-1-phosphate? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic

[Histonet] Glassware Cleaning again

2014-08-15 Thread Laurie Colbert
Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323)

[Histonet] tissue falling off slides

2014-08-15 Thread Burnett, Brandy
Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much

[Histonet] antibody suggestions CD8, FAP, Cd11b, CD11c

2014-08-15 Thread Mesru T
Dear Histonetters, I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on

[Histonet] RE: Tissue falling off

2014-08-15 Thread Terri Braud
Hi - Just a couple of suggestions - I've seen this periodically crop up during the years. There are a couple of things to consider: Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. Be

Re: [Histonet] RE: Glassware Cleaning again

2014-08-15 Thread darkdaym
Brian, et.al., It's not a typo or and an error. It's one might call a variant spelling. The Merck Index lists Bromcresol Purple, CAS Number 115-40-2. Below is what Sigma lists with the same CAS number. I've noticed the prefix Brom or Bromo on the name of several dyes. Either way, it's the

[Histonet] Re: antibody suggestions CD8, FAP, Cd11b, CD11c

2014-08-15 Thread gayle callis
You Wrote: I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on