Hi All,
I am looking to purchase equipment to set up a new AP laboratory.
Looking at second hand quality Leica :
Tissue Processors
Coverslippers
Stainer - preferably ST4040
Any brand:
Embedding Centres
Low profile Manual microtomes
Water Baths
Cold Plates
Anything to do with
Assuming that the 'crust' is a precipitate, what is it soluble in?
Water, alcohol, dilute acid or base?
It shows up if you just run the peroxide+DAB rxn?
Geoff
On 9/19/2014 8:14 AM, Donna Maney wrote:
Hello,
I've been doing IHC on free-floating brain sections for about 15 years using
the
Hi, I need to work up the Dako CMV for a project. I have an Autostainer Plus
and an Autostainer Link 48. Would someone be willing to share their procedure
with me?
Many Thanks,
Kathryn Stoll
Supervisor Histology
Clinical and Translational Research Core Lab
Medical College of Wisconsin
9200
Hi Donna,
Interesting conundrum! A few questions:
- Do you see it in tissues that have not undergone any staining, just floated
in buffer and coverslipped?
- Have you tried wet mounting them after staining and prior to dehydration and
clearing to see if the artifact is there?
- Have you
I use the Dako CMV concentrated Ab with a 1:6000 titer using the Mouse LP and
AR 6.0
Hope this help, good luck!
Cindy
Cynthia Bulmer HT(ASCP)QIHC
IHC Supervisor, CTPL
Waco, TX
From: Stoll, Kathryn kst...@mcw.edu
To: histonet@lists.utsouthwestern.edu
You will need to totally fix the heads over a longer period of time.
Perfusion is ideal if you can to that, followed by immersion fixation for a
week. Otherwise, simply remove the head and immerse into a very large
volume of NBF.If you only want to see is the skull and brain, you can
Teri makes an excellent point about higher alcohols causing
precipitation of phosphate salts.
Geoff
On 9/22/2014 1:17 PM, Teri Johnson wrote:
Hi Donna,
Interesting conundrum! A few questions:
- Do you see it in tissues that have not undergone any staining, just floated
in buffer and
