[Histonet] FW: Formalin use in grossing

2014-10-17 Thread Santiago, Albert
Hello again my fellow histonetters, I was hoping someone could give me their thoughts on this issue: We receive Derm biopsy specimens in formalin containers. We gross the specimen and place the cassettes with the tissue in it in a tray with 10% formalin. Although we have grossing tables with ex

[Histonet] reduce Paraffin fluorescence

2014-10-17 Thread avistarop
Hello I am writing to request if you could send me the protocol of Inmunohistochemical staining for reduce the Paraffin fluorescence. I saw that the people use evans blue, but I don´t know how can I do? Thank you Good weekend ALDANA VISTAROP Laboratorio Biologi­a Molecular, Servicio de

RE: [Histonet] Histology Tracking systems

2014-10-17 Thread Joelle Weaver
I have used a keyboard at embedding, but no mouse. The other tracking uses I have seen use scanning of bar codes or QR codes not keyboard or mouse entry. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: donna.wil...@baylorhealth.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu

[Histonet] RE: processing and staining preserved specimen

2014-10-17 Thread Schade, Adelle
Thank you Tom and Pat- Here are the biopsy sizes: I have my classes experimenting with specimen size to try to find what will work best for our situation. My 1st block class has 2mm biopsy cores, 2nd block has 4mm biopsy cores and 3rd block has 6mm biopsy cores- each about 2- 3 mm thick. I w

[Histonet] RE: processing and staining preserved specimen

2014-10-17 Thread Truscott, Tom
Hi Adelle, Biopsy sizes will matter, but you might shorten some of these times, but be sure to add at least one more Histoclear to make sure there is no alcohol in it and at least one more paraffin to make sure there is no histoclear carryover.If you go back to 70% at any time you will have to s

[Histonet] RE: processing and staining preserved specimen

2014-10-17 Thread Marcum, Pamela A
First of all THANK YOU for getting our field into a high school and teaching the kids. This is a very good thing for all of us in the field. Could you give us an idea of the size of the specimen you are attempting to process? A great deal depends on the tissue size as to how long it can be l

[Histonet] processing and staining preserved specimen

2014-10-17 Thread Schade, Adelle
Hello everyone, I have been following and learning from this listserv for the past two years, I have enjoyed all of the advice this forum presents. This is my first inquiry for the group. I am a high school Anatomy and Physiology teacher and a grad student at Thomas Jefferson (Philadelphia, PA

[Histonet] RE: Histology Tracking systems

2014-10-17 Thread Bernice Frederick
Our tracking system has everything there. We can create templates for various scenarios based on what is needed. The barcodes are generated by the system. It's a complicated system and takes a little time for data entry and such, but it does work. Our microscopy area is not set up in it as of ye

[Histonet] Reticle Stain

2014-10-17 Thread Bharti Parihar
Anyone know how to troubleshoot this Retic issue? The stain result is not the usual continuous silver stain reticulin fibers. Instead the fibers are more like dashed lines and the fibers are not visible throughout the entire tissue, especially edges. Ventana benchmark machines being used with thei