I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Friday, 14 November 2014 7:29 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Processed blocks before decaling Hi I have found that blocks that were insufficiently decalcified, responded best when taken back to formalin left in buffered formalin for 24 hrs and then decalcified. If you take them back to water then decalcify them, decalcification will cause more damage to the tissue then if they spend some more time in formalin. You can also put them through the clean cycle to take them back to water. Gerard Spoelstra Veterinary Histology Murdoch University -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lori.dis...@hcahealthcare.com Sent: Friday, 14 November 2014 1:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processed blocks before decaling Hello, We had several tissue cassettes put on the processor before they were decaled. Can I just melt the blocks, run thru xylene, alcohol & water. Put in decal and re-process? Thanks in advance for your expert advice! Lori Disher lori.dis...@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
