[Histonet] competency checklist

How are laboratories handling the competency checklist? Do you have one list that is all inclusive or blank forms and fill in with the competency observed? Thanks, Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwes

[Histonet] PgR Negative Cases for 2014

Did anyone else see an increase of PgR Negative cases early in 2014? Seems like there was an increase in the % of cases that were ER + but PgR negative. We saw an increase in cases particularly from Jan to Jun30th. Any thoughts? Thanks in advance, Michael Ann Michael Ann Jones, HT (ASCP) Histolog

Re: [Histonet] TRAP staining protocol troubleshooting

That's interesting because I buffered it to 4.7-5.0..that's actually a huge difference.does that have to do with he Fast Red approach? Sent from my iPhone On Nov 17, 2014, at 2:40 PM, "Patsy Ruegg" mailto:prueg...@hotmail.com>> wrote: Actually trap will work if you pre incubate it in 0

RE: [Histonet] Bone Marrow processing using Immunocal

I use a platform shaker and slosh the sample around while it is in decal solution at RT, just make sure it is well fixed before you decal, Immunocal is 5% formic acid decal solution it is not a fixative and it is a bit slower than some of the rapid decal reagents out there but is much better for

RE: [Histonet] Decalcified Bone Clearing time

Make sure that you fix well in formalin before you decal (what are you using?), then I rinse off the decal solution (I use formic acid) and put them back into formalin before putting on the tissue processor for a longer than usual schedule and especially give them longer time to infiltrate in pa

RE: [Histonet] TRAP staining protocol troubleshooting

Actually trap will work if you pre incubate it in 0.2M Tris buffer Ph9 (use sodium hydroxide to pH) for 1 hour at 37dc then proceed with the trap stain without rinsing. Check the stain in 20 min as it develops quickly after this. This protocol was given to me by the late great Hermina and it w

[Histonet] MOHS Technician Job in Arizona

Hello, I have a MOHS Technician Job in Arizona for Full Time/Direct Hire. Please message me for more details and the job description. Thank you, Melissa Owens (Phelan) Allied Search Partners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Laser Capture Microdissection Instrument

We are in the process of purchasing a LCM system, I'm interested in your opinions of Leica and Arcturus. Regards, Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23501

[Histonet] New Position Available - Field Support Scientist: Molecular

Hi All, This is an exclusively retained opportunity with a world leader in cancer diagnostics. Our client has acquired a company that specializes in molecular FISH probes, and they have recently opened an opportunity on the West Coast for a Field Support Scientist. The ideal candidate will be cu

[Histonet] Bone Marrow processing using Immunocal

I am looking for a Bone Marrow procedure that uses Immunocal as the decalcifier. I am particularly interested in how long they fix in Immunocal and if heat is used in any way . Our lab just started using this product because we are under the impression that it gave better ISH results. Thank-you a

[Histonet] Decalcified Bone Clearing time

Hey everyone! I'm having some issues about clearing the bone tissues after they get done dehydrating from decalcification. I know that over clearing the bone tissues in Xylene for too long can cause them to become very brittle and I'm afraid that that was what was happening. The bone blocks ar

[Histonet] TRAP staining protocol troubleshooting

Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain

[Histonet] ER, PR, HER2 CARGES

Hi Guys, Please can you guys tell me the CPT Codes for ER, PR and HER2? Thanks for your anticipated cooperation. Thanks, Banjo == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged inf

[Histonet] Histology Supervisor job opening near Buffalo, NY

We are currently recruiting for a client in the Buffalo, NY area for a Histology Supervisor with a valid NY lab license and 6 years of experience including experience as a leader. Interested candidates please forward an updated resume to bran...@alliedsearchpartners.com for review. To view a comp

[Histonet] FW: Job Opportunity

Hi Histonetters!! Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546 Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture co

[Histonet] Tissue Processors

We are looking to purchase a new tissue processor for our tissue core. The workload is quite light. Can anyone recommend a small, reliable processor ? Thank you, Valerie Ratliff BS HT(ASCP) Wayne State University Detroit, Michigan --- Confidentiality Notice: This email message, includi

RE: [Histonet] Re: Acetone fixation

We use acetone to denature fatty tumors without any problems. They sit in it for at least half an hour. I don't rinse them. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun..

[Histonet] HistoPro 150 Embedding Center

Hi Everyone: Has anyone had experience with the Rushabh HistoPro 150 embedding center? Could you share your thoughts and opinions about this machine? Thank you, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for th