Joanna
We are a research lab and have been using denatured alcohol for years, since
1998 we also recycle and have not seen any impact on our IHC staining.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
Hello,
Our lab is considering switching to denatured alcohol as a cost saving
initiative.
Is anyone using denatured alcohols and performing subsequent Class II IHC
testing with no impact to results?
Thank-you,
Joanna
Joanna Bartczak
MLT II - Immunohistochemistry
Calgary Laboratory Services
40
Hello again everyone,
I have a researcher requesting IHC staining for Rat Neutrophils. I've found a
few papers on PubMed and a few antibodies that are specific for Rat
neutrophils. The tissue being used is FFPE Rat pancreas and lungs. We are
specifically looking for Rat neutrophils. There was n
Have you checked lot numbers and expiration dates? I have only seen issues
with slides that are stored in a very humid place or where the temperatures
vary greatly over a year in storage. We use the ThermoFisher Colorfrost
slides and have no problems here or where I lived in Pennsylvania.
Have some cloudy Fisher Superfrost plus slides. Sometimes they're cloudy even
from boxes that are still in the plastic wrapping. Does the wisdom of the
collective histonet think these slides are okay to use or should be tossed?
Thanks!
Michelle (Shelly) Aono
~~~
Research Asso
Hello Histonetters,
Does anyone have a spare cassette processing tray (with or without the lid)
for the VIP 2000/3000 that they would be willing to part with for a
reasonable price- or know where I could get one for a reasonable price? We
have searched around and most options we have found cost a
One of the pathologists here wants to but a shaker to put the containers with
formalin and tissues on. Is this a feasible idea I've told them don't put the
formalin tissues in the cooler and don't put the whole organ in the bottles
Roberta Horner
Animal Diagnostic Lab
Penn State University
_
Great advice, Teri!
While I've not had the same request for the DNA identity test, I've had
a similar circumstance with a request for "routine" testing of a
specimen type that was beyond the scope of what our pathologists thought
as necessary diagnostic or prognostic testing. Our response was, and
