[Histonet] Masson trichrome and H and E

Hello! I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive??? Also, the people I was going to borrow their reagents from said their aniline blue is not very good

RE: [Histonet] Masson trichrome and H and E

Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around $60.00. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Tuesday, March 03, 2015

[Histonet] Lynch syndrome

I know it is a long-shot, but does anyone know of a TMA made with known Lynch Syndrome cases? We have been asked to validate MSI antibodies for evaluating CMMRD (Constitutional Mismatch Repair Deficiency) in pediatric brain tumors and don't have access to Lynch Syndrome in our pediatric

[Histonet] New York State Histotechnological Society Annual Meeting

The 2015 NYSHS Annual Meeting will be held on Saturday the 18th of April, 2015 at the Holiday Inn Express Suites Latham in Latham New York. Check out our website for more information. http://www.nyhisto.com/ Sarah Mack ___ Histonet mailing list

RE: [Histonet] Masson trichrome and H and E

We have been very satisfied with special stain solutions from Poly Scientific (http://www.polyrnd.com/products/stains/general/aniline-blue---masson's-trichrome.aspx). We get an 8oz bottle for under $30. Very good customer service too. Toni -Original Message- From:

[Histonet] Histology Supervisor and Bench histotech opportunities in Alpharetta, Ga.

A growing Pathology laboratory in Alpharetta, Ga is looking to bring some fresh faces to their Histology department. They are currently looking to hire a new Histology Supervisor, and a bench Histotechnician. ASCP certification preferred. This is a permanent, full time opportunity. Please

[Histonet] Dako Coverstainer

Good afternoon all! I would like to hear back from those of you who have the Dako Coverstainerpros and cons! Thanks much! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov

[Histonet] IHC work flow

I would love to hear about your IHC department or lab. We are looking to changing our work flow, and sometimes it's difficult to know if you are trying to perform miracles! These are the questions I have: 1. What is your volume of IHC slides/month? 2. How many antibodies do you

Re: [Histonet] IHC work flow

Clare: I think that in order to benefit the most from potential answers, you should have first offered the information from your lab for each of the questions, along with your TAT.By doing so those willing to offer their information on the subject would have a better idea if your lab is

[Histonet] Frozen section calculations

Hi All, When you receive multiple frozen sections on the same patient, does the pathologist call each one and then document the time for each one, or do they call in 'batches' and the time from receipt to call is divided by the total number of frozens done in that time frame for that patient.

AW: [Histonet] Masson trichrome and H and E

Hi! For lacking Eosin-staining check the pH of the solution. It should be 4-5. With some drops of acetic acid you may recover the staining-action. Gudrun = = = On 03/03/15, Emily Brown talulahg...@gmail.com wrote: Hello! I am doing Masson trichrome manually (not with a machine) and I just

Re: AW: [Histonet] Masson trichrome and H and E

Emely: To your initial e-mail the kit is so expensive because it incorporates a salary component for the preparation of the solutions + any and all enormous profit margin the manufacturer wants to add.I never/ever used kits, always used solutions prepared from scratch in my lab and the savings 

RE: [Histonet] Masson trichrome and H and E

Hi Emily, My $0. 02 by saying it's a challenge for research groups, like ours, to keep quality stains that are not used everyday/week/month. I agree with your intuition. Buy or make new eosin. As for stocking eosin (or hematoxylin), I buy premade by a reputable vendor and always keep an

[Histonet] Microscopy training courses or certification programs

Hello Histonet, I am curious if anyone can recommend a good microscopy training course or certificate program? I'm looking primarily for training on light microscopes. Thanks, Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National

[Histonet] Differentiating mycobacteria from Nocardia

Hello, Could anyone provide a staining protocol that will differentiate mycobacteria from Nocardia in tissue sections? The bacteria were both acid-fast-positive by the Ziehl-Neelsen stain. Thank you! Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E.

[Histonet] HT vacancy

We have an immediate opening for a registered HT. This would be a great opening for a newly registered tech as you would have the opportunity to learn and perform all aspects of histotechnology, from routine microtomy to electron microscopy, from special stains to IHC/ISH/IF and enzyme

Re: [Histonet] Masson trichrome and H and E

If you can't get two colours with HE, don't expect to get the colour scheme right with Masson's trichrome, which needs more skill. If you are hoping to show basement membranes in the kidney, you would do better to use a technically simpler staining method such as picro-sirius red or periodic

[Histonet] Histotech coverage needed in Milwaukee, WI

Help needed in covering a histotech's vacation in dermatopathology lab in downtown Milwaukee, WI. We need about 4 weeks' of coverage per year but one tech would not have to do all of it; even a week or 2 would be helpful. If interested, please respond to: Matthew Fleming, MD Fleming