Also check the gelatine - is it a new batch?
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh
[amurv...@advancederm.net]
Sent: Friday, 3 April 2015 6:01 AM
To: Jeff Halstead;
Indirect IF
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: lseb...@uwhealth.org
To: pat...@gmail.com; histonet@lists.utsouthwestern.edu
Date: Thu, 2 Apr 2015 12:25:05 +
Subject: RE: [Histonet] C3d?
CC:
Cleveland Clinic does it by IHC and I think also by IF.
Linda A. Sebree
I work in a small veterinary lab and when I do the Warthin Starry stain I mix
all the reagents in never used before disposable plastic beakers and I stain
the slides in an un-used slide mailer.
Roberta Horner
Animal Diagnostic Lab
Penn State University
-Original Message-
From:
We always acid clean before doing any silver stain. No metal
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu
The reference within a reference from Ray is A Chimeric Form of
Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1β,
TNF-α, PTH, PTHrP, and 1,25(OH)2D3 . Sean Morony et al . J Bone Mineral
Res V 14, pp 1478-1485.
However, the formic acid decalcification method is
Hello Netters, plus Matt and Keith ;-)
I'm picking the collective brain of the Histonet...
I've found some literature that suggests that up to 50% of preeclamptic
placentas that resulted in a negative outcome have diffuse C4d staining
when using IHC.
Has anyone tried C4d IHC or IF on
Hi all, as usual Gayle was right on. Use a buffered (more gentle) formic acid;
not just formic acid per se of any water diluted concentration. For end point
testing, critical, we used a radiograph machine instead of chemical endpoints
which is also fine;we just had access to a lot of
Cleveland Clinic does it by IHC and I think also by IF.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From:
I always used to rinse the containers I used with alcohol and let them dry
before doing the stain. Some people use an acid alcohol rinse. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff
Hi Sheila- I received a reply from BioCare so I am passing the information
along. Jeff.
-Original Message-
From: Dax Arguello [mailto:dargue...@biocare.net]
Sent: Thursday, April 02, 2015 11:46 AM
To: Jeffrey Robinson
Subject: RE: [Histonet] Need Antibody
Jeff,
We do have the
Hi Histonetters,
I have a question to ask if you don't mind. Can TRAP Histochemical staining be
performed after decalcifying with formic acid? Any tricks of the trade, etc?
If anyone has any experience or references that they could point me to, I would
greatly appreciate it. Thanks in
Does anyone currently have an interface between the Leica Bond stainer and
Cerner CoPath plus? How does it work? Cost?
Thank you
Nancy Schmitt MLT, HT(ASCP)
NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if
Debbie
In our hands it has not worked on formic acid decaled samples, EDTA decal only.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
March
Hello All,
I know each state's EPA laws regulate what can be done with the waste paraffin
generated during embedding and processing, but I was wondering: how do you
dispose of your paraffin waste?
Ours is thrown away in biohazard bags and incinerated, though by our state law
(OH),
I agree with Liz. EDTA.
--
Vicki L. Kalscheur
School of Veterinary Medicine
Comparative Orthopaedic Research Laboratory
2015 Linden Drive
Madison, WI 53706-1100
Phone: 608-262-8534
kalsc...@vetmed.wisc.edu
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In our hands we have never had TRAP success on Formic acid decalcified tissue.
Sarah Mack
University of Rochester Medical Center
Center for Musculoskeletal Research
Histology, Biochemistry, and Molecular Imaging Core
601 Elmwood Avenue
Box 665
Rochester, NY 14642
(585)-273-3901
Debra,
it appears most of the histology world disagrees with me but I stand by my
post. If TRAP didn't work with formic acid in our hands, a major
pharmaceutical treatment wouldn't be ready to help women with post-menopausal
osteoporosis.
Ray
- Original Message -
From: Sarah Mack
Hi Debra,
My experience differs from Elizabeth and others. Indeed have made thousands of
mouse bone preparations with formic acid decaled sections. Here is an article
that didn't copy paste so well:
RANK is the intrinsic hematopoietic cell surface
receptor that controls
http://www.novusbio.com/Bartonella-henselae-Antibody-H2A10_NB600-1208.html
CN
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, April 02, 2015 10:48 AM
To: Histonet
Subject:
Hi Sheila- I received some information from BioCare last year that they were
releasing a Bartonella (ASR) antibody. I just checked their website and I did
not see it listed but you might want to check with them (www.biocare.net).
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab,
Hi, All-I am currently experiencing problems with our warthin-stary silver
stain. The finished product has silver particulates all over the empty slide
and deposited on the tissue making the stain very difficult to interpet. Any
ideas would be very helpful. thanx
Good afternoon Histonetters!
Does anyone out there know of a good antibody for Bartonella? I would
appreciate any information you could give me. Thank you for your knowledge and
willingness to share information.
SheilaKnoxville, TN
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