FW: [Histonet] Re: Pam Marcum colleague losing bone sections from slides

2015-04-07 Thread Carol Fields
-Original Message- From: Carol Fields Sent: Tuesday, April 07, 2015 3:37 PM To: 'Cooper, Brian' Cc: 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Re: Pam Marcum colleague losing bone sections from slides I've used that also many times. It really does work. Carole F

RE: [Histonet] Re: Pam Marcum colleague losing bone sections from slides

2015-04-07 Thread Cooper, Brian
Have you ever tried blotting your slides dry before putting them into the oven? The veteran who taught me this trick used it on brain tissues from the Coroner's office (in the early 80s--don't wanna offend anyone) which were grossed very thickly and were always poorly processed. She never use

RE: [Histonet] type IV collagen antibody

2015-04-07 Thread Elizabeth Chlipala
Amy It’s been some time but we have had success in the past with the following antibodies – the SouthernBio and Abcam antibodies listed below Antibody Vendor Catalog Number Clone Host Hu Rat Rab Ms Pm Sh Gt Pig GP Dog Comments Collagen IV SouternBio 0340-01 UNLB Goat X

[Histonet] Re: Pam Marcum colleague losing bone sections from slides

2015-04-07 Thread Gayle Callis
>From Pam: I am currently trying to stain L6 vertebrae from rabbits. They have been decalcified and paraffin processed properly. I've tried cutting at both 5 and 10 microns and my tissue is still not sticking to my slides. I know my sectioning is fine because I'm successful with every other tissue

[Histonet] type IV collagen antibody

2015-04-07 Thread Amy Lee
Hello histonet, Could anyone recommend a type IV collagen antibody work on FFPE mouse tissue? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] test

2015-04-07 Thread Amy Lee
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[Histonet] Re: Laczko/Levai stain protocol

2015-04-07 Thread Gayle Callis
I found several pages from Hayat's book Stains and Cytochemical Staining 1993 (for EM) with original authors cited after a Google search, key words Laczko and Levai staining protocol. Go to this long link and read on hints to improve staining plus the protocol. https://books.google.com/books

[Histonet] Re: Anyone need a Benchmark Ultra?

2015-04-07 Thread Jeff McKenna
Is anyone out there looking for an extra Ventana Benchmark Ultra for IHC/ISH? I have one in good shape that is not being used anymore and I am looking to free up some space. Email me if you are interested and I'll give you more info on it. Jeff Mack dermack...@gmail.com On Tue, Mar 17, 2015 at

[Histonet] HISTOPALOOZA GEORGIA SOCIETY FOR HISTOTECHNOLOGY APRIL 17 - APRIL 19

2015-04-07 Thread Zimmerman, Billie
We are beyond procrastination, but you can still join us at HISTOPALOOZA! for an AFFORDABLE adventure. Early registration will insure your waived entrance gate fees and your registration packet. Lollygaggers are welcome for the affordable rate of $135 "all inclusive" for up to 15 CEUs (up to 1

[Histonet] Levai-Laczko stain

2015-04-07 Thread Cathie Crukley
Hi all, Does anyone have a reference or method for the Levai-Laczko stain? Thanks, Cathie Crukley London, ON This information is directed in confidence solely to the person named above and may contain confide