-Original Message-
From: Carol Fields
Sent: Tuesday, April 07, 2015 3:37 PM
To: 'Cooper, Brian'
Cc: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Re: Pam Marcum colleague losing bone sections from
slides
I've used that also many times. It really does work.
Carole F
Have you ever tried blotting your slides dry before putting them into the oven?
The veteran who taught me this trick used it on brain tissues from the
Coroner's office (in the early 80s--don't wanna offend anyone) which were
grossed very thickly and were always poorly processed. She never use
Amy
It’s been some time but we have had success in the past with the following
antibodies – the SouthernBio and Abcam antibodies listed below
Antibody
Vendor
Catalog Number
Clone
Host
Hu
Rat
Rab
Ms
Pm
Sh
Gt
Pig
GP
Dog
Comments
Collagen IV
SouternBio
0340-01
UNLB
Goat
X
>From Pam: I am currently trying to stain L6 vertebrae from rabbits. They
have been decalcified and paraffin processed properly. I've tried cutting at
both 5 and 10 microns and my tissue is still not sticking to my slides. I
know my sectioning is fine because I'm successful with every other tissue
Hello histonet,
Could anyone recommend a type IV collagen antibody work on FFPE mouse
tissue?
Thanks in advance,
Amy
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I found several pages from Hayat's book Stains and Cytochemical Staining
1993 (for EM) with original authors cited after a Google search, key words
Laczko and Levai staining protocol. Go to this long link and read on hints
to improve staining plus the protocol.
https://books.google.com/books
Is anyone out there looking for an extra Ventana Benchmark Ultra for
IHC/ISH? I have one in good shape that is not being used anymore and I am
looking to free up some space. Email me if you are interested and I'll give
you more info on it.
Jeff Mack
dermack...@gmail.com
On Tue, Mar 17, 2015 at
We are beyond procrastination, but you can still join us at HISTOPALOOZA! for
an AFFORDABLE adventure. Early registration will insure your waived entrance
gate fees and your registration packet. Lollygaggers are welcome for the
affordable rate of $135 "all inclusive" for up to 15 CEUs (up to 1
Hi all,
Does anyone have a reference or method for the Levai-Laczko stain?
Thanks,
Cathie Crukley
London, ON
This information is directed in confidence solely to the person named above and
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