Garrey,
We add to water bath 3-4 drops of Tween 20.
It is decrease the surface tension and small debrises of wax and tissues.
Also sections better become flat.
Adding of Tween 20 is necessary for all type of sections: for HE, HC, IHC.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
