[Histonet] Alcian blue (was Suggestions for staining ground substances ...)

Dear Histonetters, I recently replied to a question about staining extracellular ground substance with alcian blue. Here is some more information. With the alcian blue-PAS sequence it is necessary to have a good alcian blue dye - the same as or functionally equivalent to the original alcian

[Histonet] Ventana LCS (Liquid Cover Slip)

Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett

Re: [Histonet] Suggestions for staining ground substances in the Heart

Kristie, For heart you could try the elastic Trichrome, it will stain the ground substances and is faster than the Movat. If your ground substance is devoid of staining it could be the phosphotungstic acid. Is it 5%, and fresh? Are you using two changes at 5 min each? Continue with the

[Histonet] Histotech position in Tampa, FL!!

Our state-of-the-art dermatologic surgery practice in New Tampa is seeking a motivated candidate with professionalism, attention to detail, and a strong work ethic to join our team! Beautiful work environment and great benefits. Responsibilities include Mohs specimen (frozen section)

[Histonet] Looking for Opportunity in Canada

Hi, I hope you guys are doing well. I am looking for a job opportunity in Canada and I will really appreciate it if you guys could help me. I am an Histotech. With both HT(ASCP)and HTL(ASCP)Certifications. I also have both QIHC(ASCP) and QLS(ASCP)Qualifications. Thanks, Isaac

[Histonet] Distain Masson Trichrome

Could you please let me know what % of Ammonia water is used for distain Masson Trichrome? Or what % is most common used? Thank you. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458

Re: [Histonet] Ventana LCS (Liquid Cover Slip)

You certainly can air/oven dry your slides instead of running them down and coverslipping; we do that with the slides we take off in the morning when we get in. As to your problem with water/oil on your slides, something is not right in your dawn water rinsing, dehydration and/or clearing

Re: [Histonet] Distain Masson Trichrome

Usually the strong 28% is used but I always prefer using a diluted solution so I have more control on the distaining.René On Thursday, July 2, 2015 12:10 PM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: Could you please let me know what % of Ammonia water is used for distain

[Histonet] Cytologic and Frozen Specimens - IHC Techniques

Is anyone able to recommend a good reference on preparation techniques for immunostaining of cytologic and frozen specimens? I'm studying for the qIHC exam and I'm striking out with my usual sources (Carson, DAKO guide, etc.) on some of the more specific questions in these areas. Thanks! Anna

[Histonet] Humidity

I have had an issue on a couple of occasions now. Our lab gets really humid and when I have a block that needs re-embedded, the tissue becomes saturated with water and difficult to cut. The tissue seemed properly processed before, but after embedding, it almost peels out of the block. Has