[Histonet] email issues

2015-07-21 Thread Linda Margraf via Histonet
Dear Histonetters, I have heard from a couple of list members that they have been removed from the list from excess bounces and once they have gotten back on the listserv membership list, they no longer receive a copy of any message they post to the list. They use Yahoo and AOL. I contacted

[Histonet] Tissue processing validation

2015-07-21 Thread Ann Specian via Histonet
We are moving out Peloris tissue processors to another room, so we have to validate. I was wondering what other labs do as a validation for this type of move? Do you run tissue on every progam utilizing each processor for at least one of the protocols? Any help would be appreciated. thanks,

[Histonet] Tissue processing validation (Ann Specian)

2015-07-21 Thread Steve McClain via Histonet
This seems like overkill. Just moving a processor previously validated should not require re-validation, just verify the programming and make certain the paraffins are melted completely. This may not apply to all processors, but for all Sakura machines manufactured since 1980,I would not

[Histonet] Decalcification and Neutralization (Huynh,Thomas)

2015-07-21 Thread Steve McClain via Histonet
I have not found the need for neutralization. With sufficient washing, common histochemical stains including HE Gram PAS-fungus and trichrome are spectacular. Please explain your protocol for neutralization. Steve A. McClain, MD ___ Histonet mailing

[Histonet] PLA2R on paraffin sections- IHC versus indirect immunofluorescence

2015-07-21 Thread Linda Margraf via Histonet
Here is a message I am posting for Martha... My renal pathologists want to add this antibody and I want to see what methodologies everyone is using. The article I was reading mentioned both as an option.I would prefer to do IHC and run it on our Bond 3, as opposed to the very manual

Re: [Histonet] Bone histology charges

2015-07-21 Thread Shruti Shah via Histonet
Hi All, Can any one let me know general price for mouse bone decalcification, processing, microtome charges for per block or per slide. it will great help. Many thanks, NOTICE Please consider the environment before printing this email. This message and any attachments are intended for

Re: [Histonet] Tissue processing validation

2015-07-21 Thread Tony Henwood (SCHN) via Histonet
The validation would already have been done. You only need to check if the re-location has affected any equipment components. I would take a few cassettes of spare tissue and process it on your most used program and await the results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys,