Re: [Histonet] Re. Decalcification with formic acid sodium citrate

2015-07-25 Thread Merissa via Histonet
Thank you, Gayle! This is exactly what I was looking for and we are willing to make this in house. We are trying just for if acid and water, but the buffering salt should be added. I will try de calcifying mouse knees next week with this protocol. Thank you for the reference, I appreciate your h

[Histonet] Re. Decalcification with formic acid sodium citrate

2015-07-25 Thread Gayle Callis via Histonet
Merissa and Tim, This formic acid decalcifying solution is basically the classic Evans and Krajian fluid (Sheehan and Hrapchak, Theory and Practice of Histotechnology, 2nd edition, P.92). Shandon has added other ingredients for some reason, and has kept those concentrations proprietary.