Re: [Histonet] Hematoxylin Precipitate

2015-09-23 Thread Manfre, Philip via Histonet
Well Tim, In the 31 years I have been in histology, I obviously have done all lab work with my eyes closed, never looking at the equipment or solutions I have been using. I guess my stint in a high throughput contract lab taught me nothing nor did my 25 + years in big Pharma,

[Histonet] Cryostate decon ANP.23410

2015-09-23 Thread Nancy Schmitt via Histonet
Good Morning- Defrost and decontaminate with TB disinfectant weekly if used daily. How are you best managing this and what are you using to decontaminate for TB? Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally

Re: [Histonet] Cryostate decon ANP.23410

2015-09-23 Thread Michael Ann Jones via Histonet
We do maybe 11 frozens/month and so decon/defrost quarterly. Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 9/23/15, 9:33 AM, "Nancy Schmitt via Histonet"

Re: [Histonet] Hematoxylin Precipitate

2015-09-23 Thread Bob Richmond via Histonet
The alum hematoxylins I've worked with required daily filtration. An iridescent scum on top of the hematoxylin means you're overdue to filter. When you brew a fresh batch of alum hematoxylin, it's a light yellow color. As it oxidizes (slowly if you depend on atmospheric oxygen, instantly if you

Re: [Histonet] RUO expiration

2015-09-23 Thread Kiran via Histonet
Dear Experts, How do you guys determine expiration dates for RUO Abs? Thx Kiran > On Sep 23, 2015, at 10:53 AM, Chung, Fai via Histonet > wrote: > > Ventana is selling their Discovery instrument for research use only. I am > wondering if anyone know if a

Re: [Histonet] Ventana Discovery

2015-09-23 Thread Chung, Fai via Histonet
Ventana is selling their Discovery instrument for research use only. I am wondering if anyone know if a complete full validation is done on the instrument, can we use it on clinical cases as a "laboratory developed test". For antibody classified as RUO, we are able to use it on patient slide

[Histonet] Kappa/Lambda bone marrow

2015-09-23 Thread Burnett, Brandy via Histonet
Does anyone out there have a Kappa and Lambda protocol for ISH on the Ventana BenchMark Ultra? Was also wondering what type of decal should be used for ISH? Thanks! Brandy Burnett BS,HTL(ASCP) QIHC bburn...@capecodhealth.org

[Histonet] Fungus contamination on AFB slides

2015-09-23 Thread Scott, Allison D via Histonet
Hello to all in histoland. We are currently having a problem with candida on our AFB slides. We are doing the stain by hand due to the unavailability of the AFB kits from ventana. We are using bottled sterile water for rinses and the slides are not being put in coplin jars for staining.