[Histonet] Elastic Stain on Ventana BenchMark Special Stainer

2015-10-08 Thread P Sicurello via Histonet
Hello Histoes, I've got a Ventana BenchMark Special Stainer and the best I am getting so far is purple. Does anyone have a protocol they have worked out that makes the elastic fibers black? If you have a protocol will you share it with me please? Thank you, Paula UCSanDiego Health System

Re: [Histonet] Fatty breast tissue

2015-10-08 Thread Bob Richmond via Histonet
Carolyn Demarinis at Saratoga Springs NY asks: >>What are the best choices for processing fatty breast tissue when the pathologist grosses the tissue the same day it is excised?<< Breast tissue should always be grossed promptly, and placed in cassettes on the day it's excised - that's particularly

Re: [Histonet] Fatty breast tissue

2015-10-08 Thread Patrick Laurie via Histonet
The key is to make sure that you have enough formalin fixation (greater than 6 hours for ASCO/CAP standards for biomarker standards). That is a bare minimum, and if the sections are thick, usually unacceptable cutting quality will result. It would be best if the pathologist could wait at least 12

[Histonet] Fatty breast tissue

2015-10-08 Thread Demarinis, Carolyn via Histonet
What are the best choices for processing fatty breast tissue when the pathologist grosses the tissue the same day it is excised? -- This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you ar