[Histonet] C3 IF staining of FFPE tissue

Hello List, I suspect it may be a lost cause (since processing likely kills the antigen site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I know frozen is ideal but occasionally, we need to revert to the FFPE sample. Best, Kristyn

[Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016

Hi Histopeeps! What would make the perfect management position? • Is it the location? • Perhaps the type of lab? • How about the size of the staff/lab? • Maybe it’s the Hours? The Money and/or benefits? • Could it be that a particular situation is the next logical step in your career? More than

[Histonet] Issue with Leica/Jung Histoembedder

Dear all, We have a Leica/Jung Histoembedder, and it stopped working twice recently during embedding. The symptoms are: illumination light went off, hot and cold plates stopped working, the wax dispenser didn’t flow anymore, wax reservoir stopped heating. There was no error message displayed.

[Histonet] California Job Openings- UPDATE

Hello, I don't know what happened to the Laboratory Assistant link? This is the correct one. Lab Assistant- https://goo.gl/fIHSu4 Thank you, Jillian A. Russell Jillian A. Russell, HT (ASCP)CM, QIHC Histology Supervisor, Companion Diagnostics, R Diagnostics & Genomics Agilent Technologies

[Histonet] Fw: Validation

On Monday, February 8, 2016 2:37 PM, Elaine allison Hoffman wrote: Hello everyone, Would anyone be willing to share their validation protocols with me?  We are a small GI lab and we will be moving our lab to another location, into a new building.  So I

[Histonet] California Job Openings

Hello All, I currently have two job postings open, one for a Histologist and the other for a Laboratory Assistant for the research laboratory at Dako North America. Please click on the links below if interested for job details and to apply. Histologist- https://goo.gl/todRZb Lab Assistant-

Re: [Histonet] C3 IF staining of FFPE tissue (Kristyn Ferber)

Hello, Kristyn, I don't know what species you work with but I work in mouse (mouse lupus kidneys) and had good luck with Dakos C3c antibody F0201 using Dakos Proteinase K 10 minutes at room temperature. I used a unlabeled version that is not available anymore but