[Histonet] 10% NBF Question

Hello Listers, Has anyone had any problems with fixation using Richard-Allan Scientific 10% NBF? Some of our gastric biopsies are looking a little off. The rest of the biopsies fixed in the same NBF and processed on the same processor look fine. This issue would have come up in the last

Re: [Histonet] Bar Code and Tracking

I believe it will be difficult to afford the cost of Co-Path in your small lab area, unless you can attach to a bigger hospital information system contract. I suggest you look to companies that have developed products for the small to medium sized histology labs. Sent from my Windows Phone

[Histonet] Microm Block Holder

Hi Histonet- I am searching for a block holder for our Microm microtome and am wondering if anyone has one they are willing to part with or sell. Thanks in advance for your help! Erin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Herpes Zoster (VZV)

Hi everyone- Will anyone share where they get their Herpes Zoster antibody from for FFPE? I currently get it from abcam and run them on a Ventana Ultra. It seems once a year or so, the antibody just stops working and I have to call abcam and they send me a new one and it usually works. This

Re: [Histonet] Lab Coats and OCT

I am a fan of Cryo-Gel - highly viscous which facilitates easier tissue orientation. We get it from Electron Microscopy Sciences (#62806-01). Try it, you'll like it. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West

Re: [Histonet] Lab Coats and OCT

We get our freezing medium from TBS. it is called TFM (tissue freezing medium). Lately it has become much less viscous, one of the reasons I liked it was that the viscosity allowed me to orient my tissue a little easier. The packaging also changed claiming "same TBS Product, Great New

Re: [Histonet] Lab Coats (and post)

Heather, We get our disposable lab coats from Choyce: Item 6250-B $94/case http://www.choyce-products.com/our-products/personal-protective-equipment/protective-clothing/lab-coats.html Latest blog post:

Re: [Histonet] Lab Coats and OCT

Heather, We are currently using a lab coat made by White Knight, which is fluid resistant. I don't know if it's any cooler, but they do protect what you're wearing under it. Regarding the OCT. We also get our from Sakura, and have not noticed any change to the consistency. Is this happening

[Histonet] Lab Coats and OCT

Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for

Re: [Histonet] keratohyalin granules

Do you stain with hematoxylin only? or also with eosin. I think eosin is the dye, that would highlight the basic proteins in the granula. Gudrun -Ursprüngliche Nachricht- Von: Kalleberg, Kristopher via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Mittwoch, 17. Februar

Re: [Histonet] Nuclear Bubbling

Willem: Essentially incomplete deparaffination shows a different pattern and is not limited to nuclei only.Again, this is what the consensus is:1- there is ALWAYS  water left underneath the section2- that water HAS to be eliminated before the section is dried in the oven3- the best sectioning

[Histonet] Question for General Data users

Good Morning! Does anyone else use General Data for their barcode labeling system? If you do, is your operating system the HTS system or Lab Cycle? Thanks in advance! Amanda Reichard, HTL (ASCP)cm Histology Supervisor Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055

Re: [Histonet] Nuclear Bubbling

incomplete deparafinization has my voteincrease times in xylene and I bet it goes away. This happened to me when the paraffin that I had bought for years was slightly changed without notifying anyone. If I remember right the polymer was increase resulting in xylene being less effective.