What are people using for minimum fixation times in NBF 10% for tissues in a routine mixed sample load Histology Lab, on a routine VIP 5000 processor I have concerns1.) for Bio-safety,2.) Immunohistochemistry, 3.) Routine samples nuclear staining (no alcohol fixation ),4.) Sample firmness prior to grossing.
We handle approximately 6000mixed samples a year. The gamut ranges from Breast,Bowel to ECC's. The technologists have been given the responsibility within the last year for grossing or sampling smaller samples. - Bx's, skins and Hernia sacs. The Management has been more concerned about turn around times for Surgical samples, than any of the other Scientific concerns noted above. This journal article is purported to recommend minimum 24 hours fixation; Goldstein NS, Hewitt SM, Taylor CR, Yaziji H, Hicks DG. Recom- mendation for improved standardization of immunohistochemist- ry. Appl Immunohistochemistry Mol Morphol 2007; 15:124-33. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet