Re: [Histonet] Porcessing FFPE tissue without alcohol??

2016-03-25 Thread Hobbs, Carl via Histonet
Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using

[Histonet] Histopalooza III Georgia Society for Histotechnology Legacy Lodge Lake Lanier, Georgia

2016-03-25 Thread Zimmerman, Billie via Histonet
The word of the day is procrastination. Procrastination isn't so bad. You'll always have something to do tomorrow. But today is the day you need to reserve your room at the Legacy Lodge! There are 10 rooms remaining at the discounted price. Registration for classes will get you a FREE park

[Histonet] Brain IHC

2016-03-25 Thread Walter Benton via Histonet
Please see message below. Feel free to reach out directly to the Pathologist. From: Rudolph J Castellani [mailto:rudolph.castell...@med.wmich.edu] Sent: Friday, March 25, 2016 8:55 AM To: Walter Benton Subject: Another question - is there any option of embedding big brain

Re: [Histonet] Sliding Microtome

2016-03-25 Thread Walter Benton via Histonet
Thanks to those who have responded. Anyone able to share or train on techniques for free floating IHC? He needs that skill set too. -Original Message- From: Walter Benton via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, March 24, 2016 8:45 PM To: Histonet