Fix the tissue in Formalin, wash well in dw, then place very small pieces into
Osmium tetroxide solution ( std soln for TEM post-fixation)
Processing to Pwax as usual.
Basically, you will see lipids as black ( oxidised osmium)
That's the only way to demonstrate solvent- soluble lipids, using
The word of the day is procrastination. Procrastination isn't so bad. You'll
always have something to do tomorrow.
But today is the day you need to reserve your room at the Legacy Lodge! There
are 10 rooms remaining at the discounted price. Registration for classes will
get you a FREE park
Please see message below. Feel free to reach out directly to the Pathologist.
From: Rudolph J Castellani [mailto:rudolph.castell...@med.wmich.edu]
Sent: Friday, March 25, 2016 8:55 AM
To: Walter Benton
Subject:
Another question - is there any option of embedding big brain
Thanks to those who have responded.
Anyone able to share or train on techniques for free floating IHC? He needs
that skill set too.
-Original Message-
From: Walter Benton via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, March 24, 2016 8:45 PM
To: Histonet