Re: [Histonet] Porcessing FFPE tissue without alcohol??

Thanks, Liz. If you look at fat all the time, using Osmium.you then are not sure if you use K-dichromate? I am a tad confused Alsowhy not trim the block too much? Best wishes, Carl NB: Rene stated that I wouldn't be able to use any other fat stains...that's the point, Rene. I don't

[Histonet] Processing FFPE tissue without alcohol

While the tissue will still go through alcohol, have you considered preserving the fat with osmium tetroxide prior to routine processing? This turns the fat black, but it is retained in the tissue. Hi Histonet, is there a way to process tissue for paraffin embedding without using alcohol?

Re: [Histonet] Porcessing FFPE tissue without alcohol??

We use osmium post fixation to look at fat all of the time in mouse liver, nerve and muscle samples. It works well, sample size needs to be thin, samples are friable and can crack easily. We use a specific procedure for this it includes potassium dichromate I think, I'm at home but on Monday

Re: [Histonet] Porcessing FFPE tissue without alcohol??

Sudan Black reacts only with protein-combined fats.René On Saturday, March 26, 2016 11:20 AM, Joanna wrote: How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet > wrote: > > The only problem I

Re: [Histonet] Porcessing FFPE tissue without alcohol??

How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet > wrote: > > The only problem I see is that the fat will be preserved, as you wrote, as a > black osmium oxidate but you will not be able to use any "standard" fat >

Re: [Histonet] Porcessing FFPE tissue without alcohol??

The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.René On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: