Re: [Histonet] No More Blog Posts -- Over and Out!

Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact

Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet@lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been

Re: [Histonet] No More Blog Posts -- Over and Out!

I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my

Re: [Histonet] No More Blog Posts -- Over and Out!

Your response is WAY out of line. Keep it professional. Sent from my Windows Phone From: Manfre, Philip via Histonet Sent: ‎5/‎2/‎2016 12:08 PM To: Rene J Buesa Cc:

Re: [Histonet] IHC positive controls

Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air,

Re: [Histonet] No More Blog Posts -- Over and Out!

Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -Original Message- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD;

[Histonet] Fwd:

> From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-ow...@lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive

Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you VERY MUCH!René On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post

[Histonet] IHC positive controls

Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future

[Histonet] No More Blog Posts -- Over and Out!

To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just

[Histonet] PLA2R assay question

I have been asked to look into offering PLA2R on our renal biopsies and in doing some research on the subject I find that some people are staining frozen tissue via an indirect IMF procedure and some are staining ffpe tissues, also using an indirect IMF procedure.I would prefer to do this

[Histonet] open position

Hi All, We have an open position for a Histotech since one of my techs is retiring. This is a full-time position at a small community Hospital on eastern Long Island, NY. We are looking for an experienced tech to work early morning hours Monday thru Friday and no holidays. If interested,