[Histonet] exploding Nalgene bottles?

2016-06-08 Thread daniel blackburn via Histonet
We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because they are so resistant to chemicals. However, we're recently realized that the bottles grow very brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a result of a

Re: [Histonet] P16

2016-06-08 Thread Cartun, Richard via Histonet
We purchase the antibody from Ventana and run it on our Bond Max platforms. During re-optimization, we found we could dilute it 1:10 (15 minutes) following "H2" (high pH) retrieval and still get excellent results. Richard Richard W. Cartun, MS, PhD Director, Histology and the Martin M. Berman,

[Histonet] Histology Career Opportunities - Field Applications Specialist

2016-06-08 Thread Matt Ward via Histonet
Hello Histonet, Our exclusively retained client has opened a new Applications Specialist opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in cancer diagnostics and they are looking for an Appl

[Histonet] CAP

2016-06-08 Thread Bernice Frederick via Histonet
CAP Rant: 3 months to get here and still not here. Time frame should have ended May 30th. Black out days after that. Final date actually 10th. Called before Memorial day to say they couldn't come and could they come between 1-10th June. Now narrowed to this week. Not here. I am supposed to be of

Re: [Histonet] P16

2016-06-08 Thread J B via Histonet
Ventana medical systems is the only one I know of right now. On Wed, Jun 8, 2016, 10:47 AM Baldwin, Kathy via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Just saw someone reaching out for P16 was wondering if anyone would share > their protocol for this antibody we are using Ventana/Ro

Re: [Histonet] Formaldehyde Education

2016-06-08 Thread Terri Braud via Histonet
Annual Formaldehyde safety education should be part of your on-boarding process, as well as annual education, per OSHA Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 4. Form

Re: [Histonet] Formaldehyde annual education

2016-06-08 Thread Cates, Julia via Histonet
Jim, You are correct. OSHA requires annual education for any personnel that handle formalin to be educated in the hazards, proper handling, etc... This can be as simple or as involved as you want to make it but it should cover the basics at the very least. Thanks, Julia Cates, HT(ASCP)cm P

[Histonet] P16

2016-06-08 Thread Baldwin, Kathy via Histonet
Just saw someone reaching out for P16 was wondering if anyone would share their protocol for this antibody we are using Ventana/Roche and are stain is coming out weak :( Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indi

Re: [Histonet] P16

2016-06-08 Thread Dessoye, Michael via Histonet
Ventana/Roche bought out MTM which had the patent, so they're the only vendor you can get it from. Since you're using Bond you obviously don't want it in a dispenser, but you can buy their manual staining kit and just use the antibody from that. Michael J. Dessoye, M.S. | Histology/Toxicology/

Re: [Histonet] P16

2016-06-08 Thread Jeanne Blanchard via Histonet
We use the p16 from Ventana/Roche, but of course it comes in their dispenser. Jeanne Blanchard, CT (ASCP)CM -Original Message- From: Paula via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, June 08, 2016 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Hi

[Histonet] P16

2016-06-08 Thread Paula via Histonet
Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab mana

Re: [Histonet] Santa Cruz antibodies

2016-06-08 Thread Ray via Histonet
Pretty interesting Erin, thanks. I reread the Nature article I saw back in February detailing a bit about the complaints being investigated.  Among those complaints were notifications of goats with coyote bites and massive tumors (from where??).  Both of which could drastically affect polyclonal

[Histonet] Santa Cruz antibodies

2016-06-08 Thread Martin, Erin via Histonet
Hi all! Santa Cruz Biotech has to discontinue all goat and rabbit antibody production: http://blogs.sciencemag.org/pipeline/archives/2016/05/23/trouble-at-santa-cruz-biotechnology I hope you are all having a great Tuesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology a