[Histonet] Tissue controls error and appology

2016-06-16 Thread Rachel M Gonzalez via Histonet
Hi Everyone, I want to send a sincere apology to the histonet group. I realized now that I was wrong to send out this survey even if it was with the good intention to help my friend. I am sorry, I honestly did not realize the implication and how unfair this was. In the past, you have helped me

Re: [Histonet] Poor Staining

2016-06-16 Thread Debra Siena via Histonet
Hi Charles Getting three shades of pink depends a kit on your dehydration steps after eosin. Eosin is more soluble in water than alcohol so you have to watch how much water is being absorbed from the atmosphere and change/rotate alcohols when you see the last absolute turning pink, also if

Re: [Histonet] Poor Staining

2016-06-16 Thread Jennifer MacDonald via Histonet
Heat can cause poor chromatin staining. Is the two shades of eosin only on the biopsies, or all tissues? From: Charles Riley via Histonet To: histonet@lists.utsouthwestern.edu Date: 06/16/2016 11:19 AM Subject:[Histonet] Poor Staining

[Histonet] warthin-starry

2016-06-16 Thread Jeff Halstead via Histonet
Hi all thanx for the tips-I am not really allowed to use acid washes-don't have access to a reliable microwave-the stain is done by hand with me assimilating the different reagents-or a mastertech kit. The idea of the bleach causing problems is interesting-before I was told not to use the

[Histonet] Poor Staining

2016-06-16 Thread Charles Riley via Histonet
Can over processing small biopsies lead to poor chromatin staining? Also can this cause an issue with the eosin staining ( for example only getting to shades of pink instead of three)? Please give me any feedback you can -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs

Re: [Histonet] warthin-starry stain

2016-06-16 Thread Jennifer MacDonald via Histonet
are you doing a microwave method or traditional? From: Jeff Halstead via Histonet To: "histonet@lists.utsouthwestern.edu" Date: 06/16/2016 10:17 AM Subject:[Histonet] warthin-starry stain Hello

[Histonet] warthin-starry stain

2016-06-16 Thread Jeff Halstead via Histonet
Hello histonet---I am a tech in western Oregon, and I have been experiencing strange problems with the afore mentioned stain. When the stain is run a very strange grey precipitate deposits upon the slides. The deposition is not uniform across the slides and varies from slide to slide within the

[Histonet] Histology Medical Waste

2016-06-16 Thread Cassie P. Davis via Histonet
In May, Curt sent this out to Histoland: "Message: 2 Date: Wed, 11 May 2016 20:18:52 + From: Curt To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] embedding and microtomy "medical waste" Message-ID:

Re: [Histonet] Tissue Controls (Deceptive)

2016-06-16 Thread Rachel M Gonzalez via Histonet
Hi I am really sorry to everyone. You have always been so helpful to me I just wanted to help a colleague. Sorry, I am a scientist and did not realize the implication as unfair. I will not do that again. Rachel On Wed, Jun 15, 2016 at 3:52 PM, Jay Lundgren via Histonet <

Re: [Histonet] Staining stomach cells

2016-06-16 Thread Hoekert, Willem via Histonet
Maybe use Gastrin for the endocrine cells? Willem Van: Jay Lundgren via Histonet [histonet@lists.utsouthwestern.edu] Verzonden: donderdag 16 juni 2016 0:56 Aan: Judi Ford CC: histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Staining stomach