Re: [Histonet] CD34 for primate tissue

What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue.

Re: [Histonet] Transport training for formalin fixed specimens

Gareth I would think it would have more to do with OSHA than CDC - this could involve several modules. We have modules that cover the following - this is not all that we train on. If you are a small business OSHA can help out. We have utilized their services with a voluntary compliance

Re: [Histonet] Transport training for formalin fixed specimens

Hi Gareth, You will probably have to design your own Continuing Education Session. I would recommend the following topics: 1. Nature of formaldehyde - safety aspects, explanation of the MSDS. 2. What formaldehyde is used for 3. Appropriate packaging of pots containing specimens in

[Histonet] Transport training for formalin fixed specimens

Okay, is there a training course for just transportion formalin fixed specimens. CAP told me last year that anyone picking up specimens for us has to go through a training course. The inspector seemed to be more concerned with the employees knowledge of formalin and possible spills. So, does

Re: [Histonet] CD34 for primate tissue

2 more bits of information about my dilemma; I did try staining without any retrieval - no signal I am using rhesus kidney as a control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. So I know the antibodies and my

Re: [Histonet] CD34 for primate tissue

Hi Cathy, EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. Best, Eddie Martin, HTL, QIHC edmarti...@gmail.com Sent from my iPhone > On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative

[Histonet] HT grossing

Hi All- I have a couple things I am looking for assist on today: 1. Are your HT's doing frozen section cutting? Are they also doing the frozen section gross and inking? Are they staining the slides? We are interested in doing this and looking for how others do. 2. Do your HT's

[Histonet] Short term temp in Texas

Hi Histonetters -- I need a short term histologist (registered) for a three week stint in Texas.  GOOD lab, I'd go do it but the schedule doesn't line up for me :( Everything paid-- please respond via email with your resume.  Starts July 11-- Holler! ad...@fullstaff.org Cheryl Cheryl Kerry,

[Histonet] ARC immunohistochemistry- "sticky sections" in DAB

Hi Gabrielle, Are you doing floating sections? Did you cut the tissue recently? Do you notice your tissue is also "sticky"? If you do, there is a possibility that the tissue may be infected by bacteria. Try washing the tissue with PBS or TBS with 0.1% sodium azide a couple time. Sometimes it

[Histonet] Re #2: μm H staining, WHOOPS!!

Sorry about hitting send too soon. Repeat of things to try: a. Do not use regressive hematoxylin and eosin where hematoxylin can be overly differentiated i.e. removed from too thin sections. Use progressive hematoxylin i.e. Gill II or Gill III type formulation. DO NOT use acid

Re: [Histonet] 2 um sections H staining

You wrote: When I cut at 2μm my H and special stains look pale. How can I get my stains to pop or am I stuck with pale looking stains when sectioning that thin? I run manual specials and a manual regressive H For H I've tried increasing my time in hematoxylin (beyond the manufacturer

Re: [Histonet] How to get your stains more vibrant when cutting at 2μm?

Angela:"Pale" results are the trade-off for great quality very thin "2 µm" sections but you can always improve intensity somewhat .1- your "regressive" stain, if it is "modern Harris" has the inherent problem of lacking mercury chloride and it is little you can do about. Perhaps if you use

Re: [Histonet] Testicular biopsy for infertility

Dr. Rich Cartun asks: "What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you." I can

Re: [Histonet] Bouins for testicular biopsies

Richard, You wrote: What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you.

[Histonet] RELIA Histology Careers Bulletin - 6/29/2016 Sizzling new histology opportunities and Edible Firecrackers for your 4th of July celebration!

Hi Histonetters! Have a safe and Happy July 4th Weekend. I Have A Fun Idea to Share for Pool Parties and Cookouts… Sprinkle Red Pop Rocks And Blue Sprinkles On Iced Sugar Cookies To Make Edible Firecrackers!! I guarantee you will be the hit of your Independence Day Celebration! Do you have any

[Histonet] ARC immunohistochemistry- "sticky sections" in DAB

To whom it may concern, I am a undergraduate researcher at Vassar College. I am performing immunohistochemistry on mice using Arc primary, anti-mouse biotinylated IgG (1:200),ABC (Vector labs) and DAB. So far we have gotten really great staining, however the last immuno I ran, the sections, once

[Histonet] CD34 for primate tissue

Good morning my fellow Histo-netters, Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope