Re: [Histonet] semi-automated/automated TMA instrument to buy?

Hi Adriana, We had a Beecher but it acted stupid, so we got a Pathology Devices semi-automated arrayer. We typically build ~ 100 blocks per year, under a variety of study banners, so it's ideal for

Re: [Histonet] Histonet Digest, Vol 152, Issue 18

PAS crystals. The problem may not be the stain at all. occurring due to conditions to leaching of biochemical substances, in the tissue. Dirty processors, or use of KOH or acids applied to the block just before sectioning. Those may not be washed out. These crystals are especially common in

[Histonet] Fixation for FISH

Hello Histo-land, I'm going to start doing FISH in our lab, but I've never done it before. I read that the PFA fixative solution should be made fresh; however, I have some PFA that is being stored in the -80 freezer (for less than a year). Does anyone know if this would be ok to use? And

[Histonet] Mohs lab - CLIA or CAP inspections

We have a Mohs lab that is in the process of moving and will end up with a new CLIA number. The Mohs physician is more familiar with CLIA certification and would like for us to choose CLIA inspections instead of CAP inspections. I am very familiar with CAP inspections although not very

Re: [Histonet] block scrapers

Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from a hardware store. However, we moved away from scrapers and now use a heating block trimmer made for the purpose. Several vendors offer these. http://www.newcomersupply.com/product/paraffin-wax-trimmer We do all

[Histonet] Air Bubbles

Has anyone encountered the following problem. Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped.

Re: [Histonet] block scrapers

I use a single edge razor blade that I've opened boxes with and is dulled to just the right sharpness. And I wish people would quit throwing it away Roberta Horner Animal Diagnostic Lab Penn State ___ Histonet mailing list

[Histonet] Crystals in PAS

Hi, If you are having trouble with the instrument and the manufacturer isn't able to fix it, just hand stain them. PAS is really not a complicated stain. Please don't just accept underperforming equipment when we are all capable of so much more. Amos