Good charged slides or subbed slides will work.
In regards to the fixation, I personally find the morphology with 4% PFA O/N
followed by 30% sucrose better than doing them simultaneously.
Michelle
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CAP says that I need to have a procedure for Flow cytometry interpretation.
I have asked our reference laboratory for the procedure & they will not
help. Does anyone have a flow cytometry interpretation procedure that they
can share?
Sincerely,
JB
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Hi Jenny.
Can you use Pwax embedding as an alternative?
H&E will always be better on Pwax sections.
IHC will also be in many instances.
What abs are you probing with?
Single/double-labelling?
Yesyou are fixing for too long ( for frozen sectioningfor Pwax you can
fix your specimens for
