Re: [Histonet] PFA Fixed tissue not sticking to slides?

Hello Jennifer, yes over-fixation will cause adhesion problems. This applies to formalin fixed frozens as well as PFA fixed frozens. I have had good luck using chromated gelatin to coat slides. I have successfully cut and stained lung specimens left in NBF for several months. Commercially,

Re: [Histonet] Microm HM 505 E Cryostat

Thank you very much. I received a copy of the document. Johanna Haddock, BS, MT(ASCP) QA Coordinator ProPath - The Leader in Pathology Services 1355 River Bend Dr. Dallas, TX 75247 214-237-1623 214-237-1731 Fax To learn more about ProPath, please visit

[Histonet] FW: Microm HM 505 E Cryostat

Hi, Does anyone has the Manufacturer Instruction Manual for the Microm HM 505 E Cryostat that you can share with me? Thanks, Johanna Haddock, BS, MT(ASCP) QA Coordinator ProPath - The Leader in Pathology Services 1355 River Bend Dr. Dallas, TX 75247 To learn more about ProPath, please

[Histonet] Cryostat sectioning of frozen leaves

I am trying to cut frozen leaf sections at 6 and 8 microns. All I am getting is bunched up leaf. What is the trick/ I am cutting at -20 should be cutting at a lower temp and cutting thicker? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm

Re: [Histonet] productivity standards/slide

That's a good question, but tough to answer because every lab has different ways of mounting sections. I think you will have to define that for your own workflow. I've seen labs that require 30 serial sections on a slide for certain specimens, so what is that?! You could start with the one

[Histonet] productivity standards/slide

Hi everyone, What constitutes a slide? For volume purposes/tech productivity. 1 section on a slide= 1 slide 3 sections (levels) on a slide=1 slide or does it equal 3 slides? Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic NE 99th Ave Portland, OR

[Histonet] QAQC Procedure:

Is anyone willing to share a procedure for QAQC? I have a procedure that is extremely detailed and our physicans would like a shorter version. I would love to see what others have in practice if you are willing to share it is greatly appreciated. Sincerely, JB

Re: [Histonet] Pathology Ladder System

Our career ladder at UCSF HLT 1 thru 4 (Hospital Laboratory Technician) for non-histology positions. HLT-1 is entry level, could be out of high school. To move up requires more education. HLT-2 AA to BA/BA, HLT-3, -4, BA/BS used as accessioners, basic lab techs in grossing and cytology,

Re: [Histonet] Pathology Ladder System

If you don't already have it you may want to offer a shift differential for very early or late shifts and weekends. This makes it a little easier to hire for and keep people on the harder shifts. Anne -Original Message- From: Steve via Histonet

[Histonet] JOH journal collection

HI all, Is anyone interested in old issues of JOH? My collection spans 1997 - 2016. I am missing a few (~ 7). Let me know and I'll send you a list of what I have. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point,

[Histonet] **Histology Job Opportunities**

Hi there! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My team and I specialize specifically in laboratory recruiting nationwide, working directly with lab managers from different hospitals and reference laboratories across the US . We are currently recruiting for

Re: [Histonet] Pathology Ladder System

I have had the same discussion with lab administration recently. I would be interested in the same information. Aimee -Original Message- From: Steve via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 7:20 AM To: histonet@lists.utsouthwestern.edu

[Histonet] Pathology Ladder System

Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer

[Histonet] Decon Processor

Hi everyone, Our lab is getting ready to install a new processor. My question is, how do we go about decontaminating our old processor to dispose of it. The old processor is a Ventana Rennaissance. Thank you Fawn Bomar - This