[Histonet] Change in linear dimensions of soft tissues in larval insect when fixed

2016-09-16 Thread Jorge A. Santiago-Blay via Histonet
Change in linear dimensions of soft tissues in larval insect when fixed Dear Histonetters: I have nearly ca. 200 museum specimens of aquatic larval insects (1-3 cm long) that I assume were killed by dumping them in (at the minimum) 70% ethanol. In the only one case that the label states anything

[Histonet] Looking for commercial source of casein (5-10g)

2016-09-16 Thread Jorge A. Santiago-Blay via Histonet
Dear Histonetters: I need 5-10 grams of casein for a protocol I am testing. The two recommended sources only supply casein at the kg level, much more than what I need. Can someone (off the list, please) suggest me a place that may sell 5-10 grams? If you have any constructive suggestions, please

Re: [Histonet] Low volume IHC instrument

2016-09-16 Thread Eddie Martin via Histonet
Hi Gareth, If Fako doesn't suit your needs, then bio are wouldn't either. The closest analyzer with full onboard retrieval that is also more open without purchasing separate detection kits to improve quality is the Leica Bond. H pylori retrieval on Bond is only 10 mins and I think CD8 is 20 mi

[Histonet] removing araldite sections from glass slides

2016-09-16 Thread Amanda Lauer via Histonet
Good morning, everyone. We have come into possession of a collection of mouse ears that were embedded in Araldite and intended for TEM. Most of the thick sections are mounted between sheets of Aclar. However, at some point there must have been a miscommunication with technician working with the