Hello, any information on how to depolymerize bone samples that are in
Technovit 9100 Poly(methyl methacrylate) PMMA polymer?
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If it is a block issue you can also often see 'white' bits on the block
surface that correlate to the torn areas on the slide, try 'polishing' the
block at 1um until they go away, cool and then try sectioning again.
I am unsure how that relates to vacuolation - as I have usually seen that
Microtomy is so important, but often over-looked. You might try giving the
paraffin block(s) to different people and have them all cut H, and then have
your pathologist compare them. If everyone's sections are suboptimal, then the
problem is with fixation and/or processing.
Richard
Richard
Hi all,
I'm curious if anyone uses a Sakura/Tissue Tek Glas Coverslipper in their lab,
and if they use xylene/xylene based media or if it would be possible to use a
xylene substitute product on the machine?
Thanks
Tyanna Stuckey
Histology Technician
North Sound Dermatology
Further researching the topic, I've learned that there are two recently
introduced non-radioactive alternatives, Savi Scout and Sentimag. I don't
understand how they work.
Coincidentally, I received a circular in this morning's mail with
information about a continuing medical education event on
Hi,
We use a Faxitron machine in our lab.
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
Blanca
If your samples are fixed and processed properly the sections should cut
without wrinkles. We process mouse pancreas on a 30 minute cycle - 30 minutes
per station starting at 50% alcohol. We trim and soak blocks on ice and we
normally do not have issue with wrinkles.
Liz
Elizabeth
Hello Histonet!
I work in a lab that uses Histoclear as our clearing agent on our automatic
stainer. I am looking for an automatic cover slipper/cover slipping tape that
we can use with this. The thing about our lab is that it is very small and we
have limited space. If there is not something
Does anyone have a CLIA competency form for histology supervisor, histotechs,
and technical supervisors that they would be willing to share? It would be
greatly appreciated!!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
Good morning everyone!
One of my pathologists says that we are having a problem with the tissue on the
slides looking vacuolated and torn. He is convinced it is from microtomy.
Anyone have any ideas? I was thinking that it might be a processing issue.
Thanks in advance!
Erin Martin,
Hoping all are doing well. Good lab-bad lab blog.
http://www.chicagonow.com/downsize-maybe/2016/10/ben-stiller-and-psa-fly-elizabeth-holmes-and-theranos-die/
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Dear Histonetters,
Searching for a product -an enzyme- its size it is not indicated in
volume or weight. It is labelled as 25 KU. Does it mean that the vial
contains 25.000 activity units?
Thanks
Pablo
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