[Histonet] Technovit 9100 PMMA Depolymerization

Hello, any information on how to depolymerize bone samples that are in Technovit 9100 Poly(methyl methacrylate) PMMA polymer? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Vacuolated and torn?

If it is a block issue you can also often see 'white' bits on the block surface that correlate to the torn areas on the slide, try 'polishing' the block at 1um until they go away, cool and then try sectioning again. I am unsure how that relates to vacuolation - as I have usually seen that

Re: [Histonet] Vacuolated and torn?

Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing. Richard Richard

[Histonet] Sakura/Tissue Tek Glas Coverslipper

Hi all, I'm curious if anyone uses a Sakura/Tissue Tek Glas Coverslipper in their lab, and if they use xylene/xylene based media or if it would be possible to use a xylene substitute product on the machine? Thanks Tyanna Stuckey Histology Technician North Sound Dermatology

Re: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization

Further researching the topic, I've learned that there are two recently introduced non-radioactive alternatives, Savi Scout and Sentimag. I don't understand how they work. Coincidentally, I received a circular in this morning's mail with information about a continuing medical education event on

Re: [Histonet] Faxitron

Hi, We use a Faxitron machine in our lab. _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax:

Re: [Histonet] Cutting blocks for mouse tissue troubleshooting

Blanca If your samples are fixed and processed properly the sections should cut without wrinkles. We process mouse pancreas on a 30 minute cycle - 30 minutes per station starting at 50% alcohol. We trim and soak blocks on ice and we normally do not have issue with wrinkles. Liz Elizabeth

[Histonet] Small Auto cover slipper to use with Histoclear.

Hello Histonet! I work in a lab that uses Histoclear as our clearing agent on our automatic stainer. I am looking for an automatic cover slipper/cover slipping tape that we can use with this. The thing about our lab is that it is very small and we have limited space. If there is not something

[Histonet] Clia Competency Form

Does anyone have a CLIA competency form for histology supervisor, histotechs, and technical supervisors that they would be willing to share? It would be greatly appreciated!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701

[Histonet] Vacuolated and torn?

Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin,

[Histonet] Lab related blog post

Hoping all are doing well. Good lab-bad lab blog. http://www.chicagonow.com/downsize-maybe/2016/10/ben-stiller-and-psa-fly-elizabeth-holmes-and-theranos-die/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076

[Histonet] KU Size

Dear Histonetters, Searching for a product -an enzyme- its size it is not indicated in volume or weight. It is labelled as 25 KU. Does it mean that the vial contains 25.000 activity units? Thanks Pablo ___ Histonet mailing list