[Histonet] Adding glycerol to concentrated antibodies

2016-12-19 Thread Allan Wang via Histonet
Hello all, I noticed CST sells most of their antibodies with 50% glycerol and recommends -20C storage and no aliquoting. They remain in liquid form in the freezer. A little bit more difficult to pipette precisely due to the viscosity and temp difference, but it seems like a good trade-off for easy

Re: [Histonet] Histonet Digest, Vol 157, Issue 15 Kawamoto methods

2016-12-19 Thread Dorothy Hu via Histonet
Mounting media, G1 for regular application. R2 is suitable for hard tissue. The difference between the SCEM and SCEM L1 embedding medium is for different temperature? The cryofilm 2C(9) can be immersed in acetone and xylene, 2C(10) is for polarized microscope, and 3C(16UF) is good for ma

[Histonet] Peloris problem

2016-12-19 Thread Weems, Joyce K. via Histonet
Hello Everyone! Has anyone ever had a problem with a Leica Peloris, in which an incorrect reagent was pulled or missed entirely by the instrument - no user error? Thanks and Merry Christmas/Happy Holidays! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell j

[Histonet] Kawamoto protoco

2016-12-19 Thread Erickson, Jamie E via Histonet
Hello histo-neters, I was able to purchase this system many years back from Dr. Kawamoto because we had many mouse bones we wanted to do IHC, ISH etc. on. After watching the video it was clear that this was not going to be fast and easy. Our impressions were th

[Histonet] Processing time for small biopsies

2016-12-19 Thread Gareth Davis via Histonet
Hi, Recently I saw a post from someone looking for a protocol for processing small biopsies. I looked on the histonet search, but found that to be too time consuming to locate the post. Anyway, I hoping to get some opinions on good process times for small biopsies. I recently changed my paraffin

Re: [Histonet] Temp Assignment for Chesapeake Urology

2016-12-19 Thread Walter Benton via Histonet
To all: Our lab is in search of temp help to cover an extended absence by a staff member. The ideal candidate will have Prostate Grossing experience and be eligible to gross based on CLIA 88 Guidelines. In addition, this person should be a strong prostate biopsy cutter and embedder. The assign

Re: [Histonet] Kawamoto protocol online from Rowe lab

2016-12-19 Thread Colleen Forster via Histonet
Thanks Gaylethis is the system the pathologist wanted to but$3000.00 and he had no money I want to try and work with more reasonably priced ideas first.not sure I will ever get this one downI think Ill have better luck with bugs! I will watch this video and read the notes

[Histonet] Kawamoto protocol online from Rowe lab

2016-12-19 Thread Gayle Callis via Histonet
Go to this link: http://bonebase.org/bonebase/ Then click on Skeletal Phenotyping, then go to Histomorphometry to see how to use Kawamoto tape system with all the sample handling. Photos and an excellent video titled High-Throughput, mutliimage cryohistology of mineralized tissues is invalua

Re: [Histonet] High/Low thermometed

2016-12-19 Thread Jessica Riggleman via Histonet
Hi Amanda, We use T&D, they record temperature and humidity. http://www.tandd.com/product/tr7wfnw_series.html -Jessica _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General