Your concerns are reasonable. Cyto-specimens are usually fixed in alcoholic solutions not in NBF. Alcohol-fixation gives false positives in Her2. This can also be seen in underfixed tissue, that is mainly fixed by the ethanols in the processor.
Her2-protein is a normal protein on the cellsurface. FFPE treatment "turns it down" and normal amount cannot be detected. This is "negative". Only Her2 positives have abundant protein to be detected with our methods. That is why correct standardizised treatment is important to avoid "false positives" and "false negatives". Gudrun leading histotechnologist, Kepler Universitätsklinikum Linz Austria -----Ursprüngliche Nachricht----- Von: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Freitag, 6. Januar 2017 18:26 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Questions RE: "what I have found about p16" and cellblocks for controls Is there anyone in Histonet land who is using both the Optiview and Ultraview on the XT and ULTRA at the same time? I'd like to ask a few questions, please email me. We have a cytology cellblock case that came out stunningly positive for Her2 and one of our pathologist thought it would make a dynamic control if there were any specimen left to make the control block. While I am excited about the possiblity (Her2 control is hard to come by around here) some concern is expressed about using it because it is initally fixed in cytolyt before FFPE. I can argue both sides of this. I would enjoy your input on this folks. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org<mailto:cda...@che-east.org> ________________________________ From: Cassie P. Davis Sent: Thursday, January 05, 2017 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: what I have found about p16 Hi Histonet folks, Thank you for all the help, for those who are following what I have found: (1) Sigma-Aldrich does have p16 (it is also know as INKa): Anti-INKa(p16) antibody, clone 13H4.1 cat # MABE1328 or Anti-p16 Antibody, clone D25 cat#MAB4133 (2) Stacy was kind enough to share this: Hi Cassie, Are you referring to Ventana? If so, they will have 2 other kits coming out for it. They will require the use of Optiview detection or it will be considered an LDT. Just found this out from my rep. Thanks, Stacy Stacy McLaughlin, HT (ASCP) Histology Supervisor Cooley Dickinson Healthcare 30 Locust Street Northampton, MA 01060 (413-582-2019 ****Stacy, thanks for sharing! We did not get that information from our rep. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org<mailto:cda...@che-east.org> Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet