Re: [Histonet] Histonet Digest, Vol 159, Issue 20Subject: simple tests for xylene contamination and alcohol conc. in Recycled alcohol

2017-02-23 Thread Steve McClain via Histonet
Eventually your recycler retort will become contaminated. When you suspect your recycled alcohol product is contaminated by xylene, here is one fairly sensitive yet simple test. Use a 100 ml glass graduated cylinder Add 50ml recycled 95% Add 50ml water. Shake well. When xylene is present it wil

[Histonet] out with the old

2017-02-23 Thread Terri Braud via Histonet
We have just replaced our 2 old tissue processors and have one very old (1989) VIP3000 floor model, the one with the magnets. It is in working condition, but will not hold the battery charge for the backup battery. We have been using it with a UPS, for 6 runs every week. If anyone is intereste

Re: [Histonet] BRG1 IHC

2017-02-23 Thread Linda Margraf via Histonet
Richard, Propath Labs in Dallas TX offers BRG1 IHC. I have used it a few times now on brain tumor cases and it seems great. We use their immunohistochemistry services all the time. Propath.com is their website. Linda M Histonet administrator -- Message: 12 Date: T

Re: [Histonet] Xylene Free

2017-02-23 Thread Mca Werdler via Histonet
I am also interested in that i run a small lab and would love to go xylene free! 2017-02-23 12:21 GMT-06:00 Blake Taylor via Histonet < histonet@lists.utsouthwestern.edu>: > Our hospital has asked us to investigate going xylene free. Has anyone > recently made a switch to a xylene alternative an

Re: [Histonet] Histonet Digest, Vol 159, Issue 20

2017-02-23 Thread Joanne Clark via Histonet
Hi James, most processors come with wiring for hookup to an alarm system. We just purchased one from Radio shack, hooked the external wiring from the processors into it, hung it on the wall behind the units,then programmed the phone numbers to call into the alarm system. It's fairly old now, b

[Histonet] GLX Linnistainer

2017-02-23 Thread Joanne Clark via Histonet
Greetings Histonetters, we recently purchased a refurbished GLX Linnistainer that has an 8 minute stain time. I was wondering if any of you out there use the same instrument and can give me some insight on your reagent sequence and what type of Hematoxylin and Eosin your using. Thank you! Joa

[Histonet] Xylene Free

2017-02-23 Thread Blake Taylor via Histonet
Our hospital has asked us to investigate going xylene free. Has anyone recently made a switch to a xylene alternative and have you been happy with your results? We currently have 4 VIP processors and two Leica auto stainers. Thanks for your input. Blake Taylor Surgical Pathology Supervisor Le

[Histonet] 1st shift and 3rd shift openings in NC

2017-02-23 Thread Angie Laparidis via Histonet
Hello Histonet users, I wanted to fill you in on two fantastic new openings in Greensboro, NC that just came available! A private laboratory is looking for a 1st shift tech and a 3rd shift tech to fill their Full-time needs. They are offering competitive packages and are in a great location. In o

Re: [Histonet] Histonet Digest, Vol 159, Issue 20Subject: Recycled reagents in VIP processor

2017-02-23 Thread Steve McClain via Histonet
We only use recycled reagents (except 100%) on processor. We only use Fresh reagents on stainer and coverslipper. Steve A. McClain, MD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Alcoholic Eosin-Phloxine in tissue processing

2017-02-23 Thread Diane Satterfield via Histonet
I am in the process of setting up a Leica TP1020 processor. I was looking at someone else's SOP where in a 100% EtOH steps they add alcoholic eosin-phloxine. Can someone explain to me what this does and do I have to add this to one of the 100% EtOH steps in the processing. Thanks Diane ___

Re: [Histonet] ATRX Antibody

2017-02-23 Thread Kurth Virginia L. via Histonet
Hi Pam I was wondering if any reached out to you about ATRX? Our neuropathologist is asking about it. Thanks Ginny Kurth vku...@uwhealth.org Surgical Pathology Developmental Specialist University Hospital Wisconsin ___ Histonet mailing list Histonet@

[Histonet] ATRX Ab Recommendation

2017-02-23 Thread Kurth Virginia L. via Histonet
Hi Dave Did you ever receive any information on the antibody ATRX? Our neuropathologist is interested. Thanks Ginny Kurth vku...@uwhealth.org Surgical Pathology Developmental Specialist University Hospital Wisconsin ___ Histonet mailing list Histone

[Histonet] BRG1 IHC Testing

2017-02-23 Thread Cartun, Richard via Histonet
Is anyone doing clinical IHC testing for BRG1/SMARCA4? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospi

[Histonet] External alarm system for VIP 6

2017-02-23 Thread Fortune, James A. via Histonet
Hello all, We are looking to purchase an external alarm system for our 2 vip 6 processors. I called Sakura to see if they sold them or if they had any recommendations as far as which ones are compatible with the VIP and got nowhere. So I am asking all of the histonet world for any recommenda

[Histonet] Exciting Day Shift Histology Position in NJ!

2017-02-23 Thread Pam Barker via Histonet
Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me.  I am presently working with a client near Elizabeth, NJ in need of a histotech.  This is a full time permanent DAY shift position.  ASCP certification is required and 2 years of experience