Hello Histoland,
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Something new to me... A pathologist said he is getting reports that LED
illumination on newer microscopes is leading to faint or even false negatives
for congo red amyloid birefringence due to the difference in spectrum between
LED and tungsten filament lamps. Has anyone else noticed this?
Ti
Since you only have 1 slide I would recommend following your regular protocol
that you use for formalin-fixed, paraffin-embedded tissue. I would use heat
retrieval if that's what you use for FFPE tissue. However, if you use enzyme
digestion on FFPE tissue I would "not" digest your cytospin sli
Dear histonetters:
I never done ICC.
A scientist brought me a slide with Cytospined cells on for IHC.
I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.
May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE
tissue: blocks wit
Hello Histonetters,
I hope your week is off to a great start!! I know mine is!!
I want to remind you once more about the hashtag #hpd
Search the hashtag on Facebook, Instagram and Twitter to see all the fun
your fellow histotechs had on Histotechnology Professionals Day!!
I would also li