[Histonet] Day Shift Histotech Job in Southern CA

2017-03-14 Thread Melissa Owens via Histonet
Hello Histoland, I have a Day Shift Histotech opportunity in Southern California for full time permanent employment. Please contact me directly for more details. Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572

[Histonet] Congo red and LED illumination problem?

2017-03-14 Thread Morken, Timothy via Histonet
Something new to me... A pathologist said he is getting reports that LED illumination on newer microscopes is leading to faint or even false negatives for congo red amyloid birefringence due to the difference in spectrum between LED and tungsten filament lamps. Has anyone else noticed this? Ti

Re: [Histonet] Immunoperoxidase Protocol on Cytospin

2017-03-14 Thread Cartun, Richard via Histonet
Since you only have 1 slide I would recommend following your regular protocol that you use for formalin-fixed, paraffin-embedded tissue. I would use heat retrieval if that's what you use for FFPE tissue. However, if you use enzyme digestion on FFPE tissue I would "not" digest your cytospin sli

[Histonet] Immunoperoxidase Protocol on Cytospin

2017-03-14 Thread Margaryan, Naira via Histonet
Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks wit

[Histonet] Hope your Histotechnology Professionals Day was Great! Check out #hpd

2017-03-14 Thread Pam Barker via Histonet
Hello Histonetters, I hope your week is off to a great start!! I know mine is!! I want to remind you once more about the hashtag #hpd Search the hashtag on Facebook, Instagram and Twitter to see all the fun your fellow histotechs had on Histotechnology Professionals Day!! I would also li