[Histonet] Use of sodium borohydride in immunofluorescence

2017-03-20 Thread Lucie Guernsey via Histonet
Hi, I'm currently going over IHC SOPs that were written by lab members that have long ago moved on to other jobs. One inconsistency that I've come across is the use of sodium borohydride in immunofluorescence. Our protocols state that a '0.5% sodium borohydride solution should be used to block

[Histonet] FT EVENING HT POSTITION Delray Beach, FL

2017-03-20 Thread Kari Simeone via Histonet
Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Beach Florida Dermatology Lab. This is a permanent full time EVENING SHIFT (40 hours) position with benefits (medical/401k/vacation). THIS IS A DRUG FREE WORKPLACE. Background check,

Re: [Histonet] need help staining 120um human whole brain sections!

2017-03-20 Thread Maria Mejia via Histonet
Good morning Rene, I have worked out special staining IHC protocols to work on celloidin cut sections from 60um to 600um thick. These are free-floating human whole brainstem & whole brain sections. The IHC is amazing using single, double & triple. We save on antibodies, ABC, polymer, TSA

[Histonet] Toxicology Histology Supervisor Position

2017-03-20 Thread Oconnor, Jackie M via Histonet
Hello Histonetters - we are looking for a Histology Supervisor. If you are interested, please email me. Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] need help staining 120um human whole brain sections!

2017-03-20 Thread Rene J Buesa via Histonet
You have a special project → special tasks so your approach has to be equally special.Large brain sections are usually stained while floating but for IH with different and successive steps requiring very expensive reagents, floating sections is not well suited.You should affix the sections to