Stainer for sure.
JB
On Fri, Mar 31, 2017, 2:05 PM Walter Benton via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Patti,
>
>
> If you are able to incorporate the special stains along with the H&E on
> the automated stainer, I think that would be the best option. Based on your
> inform
Patti,
If you are able to incorporate the special stains along with the H&E on the
automated stainer, I think that would be the best option. Based on your
information you may have 120-180 slides to coverslip by hand each day.
Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urolo
Walter the staff consist of 1 Histotech and 1 lab assistant. On an average
there will be 2 special stains per block. Hope that helps.
Sincerely,
PATTI NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONF
How many slides are you producing from that number of blocks? How many techs do
you have that are able to hand stain and/or hand coverslip?
Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410
Hands down the Automatic Stainer preferred over the coverslipper. You can
do H & E & you can do the AB/PAS as well on it. No standing there hand
dipping. You can be doing something else and come back and take them off to
sit and hand coverslipp. Email me personally if you want to know which
Automa
I'm going to give my two cents here. I think you need to look closely at how
you handle these samples. First of all leeps are not small samples therefore
just because the leep has been placed in fixative upon removal and then sits in
fixative until its processed. Unless the sample is grossed
Hi Everyone,
I just wanted to get everyone's opinion. If you had to chose between buying a
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab.
Everyone's input would be greatly appreciated.
I would bet there are quite a few pathologists on this mailing list (like me)
who are here because we respect histo techs and know you have valuable
information to offer!
I agree, cautery artifact is pretty easy to tell from poor fixation, so it
should be easy to determine if that is the proble
Tim describes a problem "I have a pathologist that is not happy with the
fixation on some of our LEEP specimens."
LEEP specimens are inherently crappy, because of the cautery used to obtain
them, and the resulting cautery artifact in the specimens. In the last few
years they've turned the voltage
I agree with Rene. To discredit the pathologist theory show if all of the
specimens from the run are not fixed properly. Then show if it is just the
LEEPs. Then show if it is that particular clients specimens? Then onto that
client's LEEPs.
That should prove your problem lies with the clien
We use chux here as well. The cotton in them can bunch up, also they don't lay
completely flat. We also use the thin lab mats. Cardinal carries them as does
fisher. They can be bought in bulk, have an absorbent side and a fluid
resistant side. Since we are on a tight budget here, and studen
We use squares of absorbent paper towels to first blot excess fixative and then
place on a 2mm thickness piece of art foam.
Art foam is used as a background for
photography and slicing.
By sliding or moving the foam we position the specimen under the camera during
specimen photography. We have p
I second the opinion of Joyce. We see such effects in portio-conisations, that
are done with a thermo-electrical knife. The surface and the underlying area
show a very pink colour in HE. It can also be seen in prostata-chips. IHC on
such biopsies shows the effect of an non-stainable edge with a
Our lab is once again evaluating disposal of slides/blocks from >10 years ago.
Maintaining or donating the material do not appear to be feasible options.
Aside from PHI issues, how are labs disposing of this type of material. Do you
place the slides in sharps containers? Do you red bag the bloc
Hi there,
just to update you on the issue. Thanks to the kind and useful
suggestions from histonetes, I found the solution to my problem.
Fixation for two days in 10% formalin in absolute ethanol worked
perfectly well. Brains were well fixed, we could trimmed them, and the
fixation in alcoh
Hi everyone, Happy Friday to you all..
I am hoping to gather some information on how people bank frozen tissue for
work in research. I am trying to start a bank of frozen human samples stored in
a -80 freezer for our scientists use. Information that I'm looking for is as
follows:
1.
Aren't LEEPS done with some sort of electric method that will damage the tissue
before it even reaches formalin. I'm not positive but Google it - I believe
that might be the problem. j
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare
Tim, I have to agree with Rene that the formalin or time in formalin is
obviously not the problem - it has plenty of time in formalin (and who would
dilute it anyway?). Handling before formalin must always be determined when
problems arise. If the sample sits on a paper towel, gauze etc it does
Bravo Rene! Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology,
Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX
405-759-7513www.excaliburpathology.com
From: Rene J Buesa via Histonet
To: T H ; "histonet@lists.utsouthwestern.edu"
Sent: Friday, March 31, 2017
What you describe as a possible scenario is absolutely possible.If your PT does
not "want to hear" about it, suggest she gets a "hearing aid" or to study
something about histotechnology or even better yet, pay attention to what a
professional on the subject (you) has to say about it. You would n
Good Morning,
I have a pathologist that is not happy with the fixation on some of our LEEP
specimens. She swears its histology doing something to the specimen to cause
the tissue to look unfixed on only "part" of the LEEP specimens (all the same
client specimens). She claims we must be dilut
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