Hi Sandra,
I haven't myself particularly worked with brain and spinal cord, but majority
of my protocols in my old job used fixation in 4% PFA (24 hours at 4-8oC) and
routinely process (or transfer to graded EtOH, if not processing immediately).
However, our routine process didn't include a
Hi Jennifer,
My ISH slides (FFPE) were stored in -20oC for several months or more than
one year and the targets were less abundant. I didn't use RNAscope though,
which is more sensitive. I don't know what kind of RNA your researcher try
to locate and need to do ISH within 24 hours after cutting?
Hi Sandra,
What are the times for each processing station? Animal tissue is much
leaner, so it's easy to over process and become brittle to section.
Best,
Tina Van Meter
On Mon, Aug 28, 2017 at 11:30 AM, Sandra Cheasty via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi all,
>
Hello Histonet,
I am hoping someone can point me in the right direction to find some references
for how long FFPE slides intended for ISH can be kept before staining. I have
a researcher who swears their slides have to be stained within 24 hours of
being cut, which does not sound correct to
Hi all,
We are having difficulty sectioning mouse tissue, (brain,
spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone
had issues with paraformaldehyde fixed tissue? They were processed routinely,
starting in 10% NBF, with other tissues, and we are
Hello everyone,
Does anyone know of a company that sells the cardboard drawers
and drawer dividers for filing blocks, separate from the outer cardboard file
that the drawers slide into?
We came into possession of 25 outer files with no drawers,
which means we
