[Histonet] Cytochrome Oxidase Enzyme Histochemical Staining

2017-11-22 Thread Amy Porter via Histonet
Anyone out there doing COX enzyme histochemistry on fresh flash frozen EQUINE muscle...I have tried several methods and fibers are not differentiating the way they should be and are very faint ..any suggestions welcome!! Thanks - Amy Amy S. Porter, HT (ASCP) Michigan State University -

Re: [Histonet] Her 2 Control

2017-11-22 Thread Terri Braud via Histonet
Hey Scott - Our interpretation of CAP requirements for IHC Controls is the same as yours. Our Her2 control is a microarray with 3+ case and a piece of normal skin to serve as our negative tissue control. If you have normal breast tissue adjacent to your tumor control, that can also serve as a

Re: [Histonet] Negative IHC Control for Her2

2017-11-22 Thread Rene J Buesa via Histonet
You are right but, your point is?Regardless, the pathologist is the responsible for his/her diagnosis/interpretation and the liability is his/hers.Remember that for us histotechs, our "client" is the pathologist (always remembering the patient behind the whole process) and our essential duty is