Re: [Histonet] Histonet Digest, Vol 174, Issue 9

Jamie Watson, you need to clarify this question: >> Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor?<< 100% ethanol is perfectly OK, but it requires a lot of regulator compliance because you can drink it (though you

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Re: [Histonet] Ethanol in tissue processing

Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 and 99 percent as the last two steps in tissue processing. These ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is not allowed as denaturation agent in Europe, otherwise you

Re: [Histonet] Ethanol in tissue processing

Most places use "reagent alcohol" which, from most suppliers, is a mongrel blend of whatever is cheapest, and not even listed on the label. It's usually made up of a blend of ethanol, methanol, and isopropyl. Good luck finding what the percentages are. I've called one vendor before, and even

Re: [Histonet] Ethanol in tissue processing

We use reagent alcohol on the processor and for staining. Get Outlook for iOS From: Jamie Watson via Histonet Sent: Saturday, May 12, 2018 9:06:01 AM To: Histonet@lists.utsouthwestern.edu Subject:

[Histonet] Ethanol in tissue processing

Hi All, Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor? Jamie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu